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IGF1R/IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi)

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Based on 1 Customer Validation

IGF-I receptor (IGF1R) mediates insulin-like growth factor effects, binding strongly to IGF1. Activation triggers PI3K-AKT/PKB and Ras-MAPK pathways, influencing cell survival, protein synthesis, and proliferation. IGF1R also signals through JAK/STAT, inhibiting JNK activation. Hybrid receptors show variable binding to IGF1 and insulin. IGF1R/IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived IGF1R/IGF-I R Protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IGF1R/IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi)
  • IGF1R/IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

IGF-I receptor (IGF1R) mediates insulin-like growth factor effects, binding strongly to IGF1. Activation triggers PI3K-AKT/PKB and Ras-MAPK pathways, influencing cell survival, protein synthesis, and proliferation. IGF1R also signals through JAK/STAT, inhibiting JNK activation. Hybrid receptors show variable binding to IGF1 and insulin. IGF1R/IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived IGF1R/IGF-I R Protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

Background

The IGF-I receptor (IGF1R) is a receptor tyrosine kinase that plays a pivotal role in mediating the actions of insulin-like growth factor 1 (IGF1). It exhibits high affinity for IGF1 and lower affinity for IGF2 and insulin (INS). Upon ligand binding, IGF1R activates its kinase, leading to receptor autophosphorylation and tyrosine phosphorylation of various substrates, including insulin-receptor substrates (IRS1/2), Shc, and 14-3-3 proteins. This initiates two main signaling pathways: the PI3K-AKT/PKB pathway, which inhibits apoptosis and stimulates protein synthesis, and the Ras-MAPK pathway, promoting increased cellular proliferation. Phosphorylated IRS1 can activate the PI3K pathway, leading to downstream activation of AKT/PKB and subsequent enhancement of protein synthesis and antiapoptotic effects. Simultaneously, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc activates the Ras-MAPK pathway. Additionally, IGF1R signals through the JAK/STAT pathway, potentially contributing to its transforming activity. The JNK kinases can also be activated by IGF1R, and IGF1 inhibits JNK activation by phosphorylating and inhibiting MAP3K5/ASK1. Hybrid receptors composed of IGF1R and INSR isoforms exhibit varying binding characteristics, indicating high affinity for IGF1 and low affinity for insulin.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. When immobilized Anti-IGF1R Antibody at 0.5μg/ml (100μl/Well), can bind Biotinylated Human IGF1R His tag and the EC50 is 35.7 ng/mL.
3.Loaded Teprotumumab (HY-P99165) on AHC2 biosensor, can bind IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 4.315-10 M as determined in BLI assay.
4.Measured by its binding ability in a functional ELISA.Immobilized Biotinylated Human IGF-I R, His,Avi tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IGF-I, Fc Tag with the EC50 of 57.69 ng/mL.

MCE Validation Data

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Teprotumumab (HY-P99165) on AHC2 biosensor, can bind IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 4.315E-10 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-His
  • Accession

    P08069 (E31-N932)

  • Gene ID
    3480  [NCBI]
  • Molecular Construction
    • N-term
    • IGF-I R (E31-N932)
      Accession # P08069
    • His-Avi
    • C-term
  • Protein Length

    Partial

  • Conjugation

    Biotin

  • Synonyms

    IGF1R; IGF-I Receptor; Insulin Like Growth Factor 1 Receptor; MGC18216; Insulin-Like Growth Factor 1 Receptor; Insulin-Like Growth Factor I Receptor; JTK13; Receptor Protein-Tyrosine Kinase; CD221; Tyrosine-Protein Kinase Receptor; IGFIR; CD221 Antigen; I

  • AA Sequence

    EICGPGIDIRNDYQQLKRLENCTVIEGYLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDAVSNNYIVGNKPPKECGDLCPGTMEEKPMCEKTTINNEYNYRCWTTNRCQKMCPSTCGKRACTENNECCHPECLGSCSAPDNDTACVACRHYYYAGVCVPACPPNTYRFEGWRCVDRDFCANILSAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDGYLYRHNYCSKDKIPIRKYADGTIDIEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRPERKRRDVMQVANTTMSSRSRNTTAADTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTGYEN

  • Predicted Molecular Mass

    80.4 kDa (alpha subunit) & 25.4 kDa (beta subunit)

  • Molecular Weight

    Approximately 110-120 kDa (alpha subunit) & 52-55 kDa (beta subunit), based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Loaded Teprotumumab (HY-P99165) on AHC2 biosensor, can bind IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 4.315E-10 M as determined in BLI assay.

  • IGF1R/IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
    1/16
  • Experimental Validation Results for IGF1R/IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi)
  • IGF1R/IGF-I R Protein, Human (Biotinylated, HEK293, His-Avi) Product Image