ILKAP/PP2C delta Protein, Human (HEK293, His)
Based on 1 Customer Validation
The ILKAP/PP2C delta protein is a key phosphatase that potentially regulates cell cycle progression by dephosphorylating substrates essential for proliferation. It selectively binds to ILK and regulates cell adhesion and growth factor signaling. ILKAP/PP2C delta Protein, Human (HEK293, His) is the recombinant human-derived ILKAP/PP2C delta protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The ILKAP/PP2C delta protein is a key phosphatase that potentially regulates cell cycle progression by dephosphorylating substrates essential for proliferation. It selectively binds to ILK and regulates cell adhesion and growth factor signaling. ILKAP/PP2C delta Protein, Human (HEK293, His) is the recombinant human-derived ILKAP/PP2C delta protein, expressed by HEK293 , with C-His labeled tag.
Background
ILKAP/PP2C delta protein emerges as a key phosphatase with potential implications in the regulation of cell cycle progression by dephosphorylating substrates crucial for cell proliferation. This protein selectively associates with integrin-linked kinase (ILK), exerting modulatory effects on cell adhesion and growth factor signaling. ILKAP/PP2C delta specifically inhibits the ILK-GSK3B signaling axis, suggesting a role in restraining the pathway's activity. Such inhibition may play a critical role in impeding oncogenic transformation, emphasizing ILKAP/PP2C delta's significance as a potential tumor suppressor. The intricate interplay between ILKAP/PP2C delta, ILK, and downstream signaling pathways underscores its regulatory role in fundamental cellular processes, with implications for both cell cycle dynamics and oncogenic transformation control.
Verified Bioactivity
Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 562.344 nmol/min/mg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q9H0C8 (M1-H392)
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Molecular Construction
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N-term
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ILKAP (M1-H392)
Accession # Q9H0C8 -
His
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C-term
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Protein Length
Full Length
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Synonyms
ILKAP; ILKAP2; ILK Associated Serine/Threonine Phosphatase; ILKAP3; Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C; Truncated Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C; PPM1O; Integrin-Linked Kinase-Associated Se
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AA Sequence
MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH
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Molecular Weight
Approximately 46 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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