Copy Link Print Information

NEK9 Protein, Human (Active, ΔT346-K731, sf9, GST)

Customer Review

Based on 1 Customer Validation

The NEK9 protein is a pleiotropic mitotic regulator that oversees spindle dynamics and chromosome segregation. It phosphorylates a variety of substrates, including histones, myelin basic protein, β-casein, and BICD2. NEK9 Protein, Human (ΔT346-K731, sf9, GST) is the recombinant human-derived NEK9 protein, expressed by sf9 insect cells , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • NEK9 Protein, Human (Active, ΔT346-K731, sf9, GST) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The NEK9 protein is a pleiotropic mitotic regulator that oversees spindle dynamics and chromosome segregation. It phosphorylates a variety of substrates, including histones, myelin basic protein, β-casein, and BICD2. NEK9 Protein, Human (ΔT346-K731, sf9, GST) is the recombinant human-derived NEK9 protein, expressed by sf9 insect cells , with N-GST labeled tag.

Background

NEK9, a pleiotropic regulator of mitotic progression, orchestrates critical processes in spindle dynamics and chromosome separation. Its pivotal role extends to phosphorylating various substrates, including histones, myelin basic protein, beta-casein, and BICD2. Specifically, NEK9 exhibits phosphorylation activity towards histone H3 on serine and threonine residues and beta-casein on serine residues. The multifaceted influence of NEK9 is underscored by its involvement in the G1/S transition and S phase progression. Moreover, NEK9 plays a key role in modulating the activity of NEK6 and NEK7, enhancing their functionality by releasing the autoinhibitory functions of Tyr-108 and Tyr-97, respectively. This comprehensive regulatory role emphasizes NEK9's significance in governing mitotic processes and ensuring the precise orchestration of cellular events during cell cycle progression.

Verified Bioactivity

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The KM of NEK9 is 54.69 µM

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-GST
  • Accession

    Q8TD19 (S2-L979, ΔT346-K731)

  • Gene ID
    91754
  • Molecular Construction
    • N-term
    • GST
    • NEK9 (S2-L979, ΔT346-K731)
      Accession # Q8TD19
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NEK9; DKFZp434D0935; NIMA Related Kinase 9; MGC16714; NERCC; Non-Specific Serine/Threonine Protein Kinase; Serine/Threonine-Protein Kinase Nek9; Alternative Protein NEK9; Nercc1 Kinase; NimA-Related Kinase 8; NERCC1; KIAA1995; Nek8; LCCS10; NIMA (Never In Mitosis Gene A)- Related Kinase 9; APUG; Never In Mitosis A-Related Kinase 9; NEK8; NimA-Related Protein Kinase 9; NC

  • Predicted Molecular Mass

    91.6 kDa

  • Purity

    ≥ 80%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 1 mM DTT, 20% glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • NEK9 Protein, Human (Active, ΔT346-K731, sf9, GST) Product Image
    1/16
  • NEK9 Protein, Human (Active, ΔT346-K731, sf9, GST) Product Image