NOTCH1 Protein, Human (HEK293, Fc)

NOTCH1 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is critical for cell fate decisions. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer. NOTCH1 Protein, Human (HEK293, Fc) is the recombinant human-derived NOTCH1 protein, expressed by HEK293 , with C-hFc labeled tag.

NOTCH1 protein serves as a receptor for membrane-bound ligands, including Jagged-1 (JAG1), Jagged-2 (JAG2), and Delta-1 (DLL1), exerting critical regulatory functions in cell-fate determination. Upon ligand activation, the released Notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH, orchestrating the activation of genes within the enhancer of split locus. NOTCH1 significantly influences cellular differentiation, proliferation, and apoptotic programs. Beyond its role in angiogenesis, where it negatively regulates endothelial cell proliferation and migration, NOTCH1 is involved in thymic maturation, follicular differentiation, and cell fate selection within the follicle. Additionally, it plays a crucial role in cerebellar development by acting as a receptor for neuronal DNER, contributing to the differentiation of Bergmann glia. NOTCH1 also plays roles in postimplantation development, mesoderm development, somite formation, and neurogenesis. It interacts with various proteins, including DNER, DTX1, DTX2, RBPJ/RBPSUH, MAML1, MAML2, MAML3, SNW1, AAK1, FBXW7, SGK1, HIF1AN, SNAI1, MDM2A, BCL6, THBS4, CCN3, DLL4, ZMIZ1, MEGF10, PRAG1, PSEN1, and ZFP64, forming a complex network that modulates diverse cellular processes and regulatory pathways.

Immobilized Recombinant DLL4 Protein at 5 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human NOTCH1 Protein. The ED₅₀ for this effect is 0.1495 μg/mL.

Human

HEK293

C-hFc

P46531/NP_060087.3 (A19-Q526)

4851  [NCBI]

Partial

ARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQ

Approximately 95 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.