1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. PDGFs & PDGFRs
  4. PDGF
  5. PDGF-AB
  6. PDGF-AB Protein, Human (Solution)

PDGF-A protein is a key growth factor regulating embryonic development, cell proliferation, migration, survival, and chemotaxis, and effectively promotes mitosis in mesenchymal cells. PDGF-B protein is a key growth factor that centrally regulates embryonic development, cell proliferation, migration, survival, and chemotaxis. PDGF-AB Protein, Human (Solution) is a recombinant PDGF-AB protein expressed by E.coli without a tag.

For research use only. We do not sell to patients.

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Description

PDGF-A protein is a key growth factor regulating embryonic development, cell proliferation, migration, survival, and chemotaxis, and effectively promotes mitosis in mesenchymal cells. PDGF-B protein is a key growth factor that centrally regulates embryonic development, cell proliferation, migration, survival, and chemotaxis. PDGF-AB Protein, Human (Solution) is a recombinant PDGF-AB protein expressed by E.coli without a tag.

Background

PDGF-A protein plays a central role as a key growth factor regulating embryonic development, cell proliferation, migration, survival, and chemotaxis. PDGF-A is renowned for its effective mitotic-promoting effect on mesenchymal cells and is indispensable for various developmental processes, including alveolar septum formation, gastrointestinal development, Leydig cell maturation, spermatogenesis, and normal oligodendrocyte development, contributing to myelination of oligodendrocytes in the spinal cord and cerebellum. Furthermore, PDGF-A plays a crucial role in wound healing dynamics. PDGF-B protein is a key member of the PDGF/VEGF growth factor family, indicating its crucial role in cell signaling and tissue development. As part of this growth factor family, PDGF-B may share conserved structural and functional features with related proteins, contributing to cell growth, angiogenesis, and vascular development.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P04085 (S87-T211)&P01127 (S82-T190)

Gene ID

5154&5155

Molecular Construction
N-term
PDGFA (S87-T211)
Accession # P04085
C-term
N-term
PDGFB (S82-T190)
Accession # P01127
C-term
Synonyms
PDGFAB; PDGF-AB
Predicted Molecular Mass
14.1 kDa & 12.4 kDa
Molecular Weight

Approximately 14-20 kDa, based on SDS-PAGE under reducing conditions.

Purity

≥ 95%, as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 4mM HCl.

Endotoxin Level

<0.1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

PDGF-AB Protein, Human (Solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PDGF-AB Protein, Human (Solution)
Cat. No.:
HY-P704705
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