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Pentraxin 3/TSG-14 Protein, Human (HEK293, His)

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The Pentraxin 3/TSG-14 protein is a member of the pentameric protein family. It is a multifunctional protein that plays a regulatory role in inflammation. It can play the role of heart protection and anti atherosclerosis by regulating inflammation. Pentraxin 3/TSG-14 Protein, Human (HEK293, His) is the recombinant human-derived Pentraxin 3/TSG-14 protein, expressed by HEK293 , with C-10*His labeled tag and with natural variants A48D mutation.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Pentraxin 3/TSG-14 Protein, Human (HEK293, His)
  • Experimental Validation Results for Pentraxin 3/TSG-14 Protein, Human (HEK293, His)
  • Pentraxin 3/TSG-14 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The Pentraxin 3/TSG-14 protein is a member of the pentameric protein family. It is a multifunctional protein that plays a regulatory role in inflammation. It can play the role of heart protection and anti atherosclerosis by regulating inflammation. Pentraxin 3/TSG-14 Protein, Human (HEK293, His) is the recombinant human-derived Pentraxin 3/TSG-14 protein, expressed by HEK293 , with C-10*His labeled tag and with natural variants A48D mutation.

Background

The Pentraxin 3/TSG-14 Protein, a member of the pentraxin protein family, is encoded by this gene. Its expression is induced by inflammatory cytokines in response to inflammatory stimuli, particularly in various mesenchymal and epithelial cell types, including endothelial cells and mononuclear phagocytes. This protein plays a crucial role in promoting fibrocyte differentiation and is actively involved in regulating inflammation and complement activation. Additionally, it contributes to angiogenesis and tissue remodeling. Notably, Pentraxin 3/TSG-14 serves as a biomarker for several inflammatory conditions, reflecting its potential clinical significance. The gene exhibits biased expression, with heightened levels in the bone marrow (RPKM 32.2), appendix (RPKM 24.8), and six other tissues, highlighting its specific involvement in various physiological contexts across multiple organs.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Anti-TSG-14 at 1 μg/mL (100 μL/well) can bind TSG-14. The ED50 for this effect is 0.04-0.1 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Anti-TSG-14 at 1 μg/mL (100 μL/well) can bind TSG-14 , The ED50 for this effect is 0.04374 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    NP_002843.2 (E18-S381, with natural variants A48D)

  • Gene ID
    5806  [NCBI]
  • Molecular Construction
    • N-term
    • TSG-14 (E18-S381, A48D)
      Accession # NP_002843.2
    • 10*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PTX3; Long Pentraxin 3; Prev. TNFAIP5; Pentaxin-Related Gene, Rapidly Induced By IL-1 Beta; TSG-14; Tumor Necrosis Factor, Alpha-Induced Protein 5; Tumor Necrosis Factor-Inducible Gene 14 Protein; Tumor Necrosis Factor-Inducible Protein TSG-14; Tumor Necr

  • AA Sequence

    ENSDDYDLMYVNLDNEIDNGLHPTEDPTPCDCGQEHSEWDKLFIMLENSQMRERMLLQATDDVLRGELQRLREELGRLAESLARPCAPGAPAEARLTSALDELLQATRDAGRRLARMEGAEAQRPEEAGRALAAVLEELRQTRADLHAVQGWAARSWLPAGCETAILFPMRSKKIFGSVHPVRPMRLESFSACIWVKATDVLNKTILFSYGTKRNPYEIQLYLSYQSIVFVVGGEENKLVAEAMVSLGRWTHLCGTWNSEEGLTSLWVNGELAATTVEMATGHIVPEGGILQIGQEKNGCCVGGGFDETLAFSGRLTGFNIWDSVLSNEEIRETGGAESCHIRGNIVGWGVTEIQPHGGAQYVS

  • Molecular Weight

    Approximately 43-47 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween 80.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Anti-TSG-14 at 1 μg/mL (100 μL/well) can bind TSG-14 , The ED50 for this effect is 0.04374 μg/mL.

  • Pentraxin 3/TSG-14 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Pentraxin 3/TSG-14 Protein, Human (HEK293, His)
  • Experimental Validation Results for Pentraxin 3/TSG-14 Protein, Human (HEK293, His)
  • Pentraxin 3/TSG-14 Protein, Human (HEK293, His) Product Image