Copy Link Print Information

PFKP Protein, Human (sf9, His-GST)

Customer Review

Based on 1 Customer Validation

PFKP protein, central to cellular energy metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This key enzymatic step regulates glucose entry into glycolysis, initiating essential biochemical reactions for ATP and metabolite generation. PFKP's importance in orchestrating this process highlights its central role in cellular energy homeostasis and broader metabolic regulation. PFKP Protein, Human (sf9, His-GST) is the recombinant human-derived PFKP protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -20°C for 1 year from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • PFKP Protein, Human (sf9, His-GST) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

PFKP protein, central to cellular energy metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This key enzymatic step regulates glucose entry into glycolysis, initiating essential biochemical reactions for ATP and metabolite generation. PFKP's importance in orchestrating this process highlights its central role in cellular energy homeostasis and broader metabolic regulation. PFKP Protein, Human (sf9, His-GST) is the recombinant human-derived PFKP protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag.

Background

The PFKP protein plays a pivotal role in cellular energy metabolism by catalyzing the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate using ATP as a substrate. This enzymatic activity marks the initial and committing step of glycolysis, a fundamental pathway crucial for energy production. Through its catalytic function, PFKP regulates the entry of glucose into glycolysis, initiating a series of biochemical reactions that contribute to the generation of ATP and other essential metabolites. The importance of PFKP in orchestrating this key step underscores its central role in cellular energy homeostasis, emphasizing its significance in the broader context of metabolic regulation.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His;N-GST
  • Accession

    Q01813 (M1-V784)

  • Gene ID
    5214  [NCBI]
  • Molecular Construction
    • N-term
    • His-GST
    • PFKP (M1-V784)
      Accession # Q01813
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    PFKP; ATP-Dependent 6-Phosphofructokinase, Platelet Type; Phosphofructokinase, Platelet; PFK-P; PFK-C; 6-Phosphofructokinase, Platelet Type; PFKF; ATP-Dependent 6-Phosphofructokinase; Phosphofructo-1-Kinase Isozyme C; Phosphofructokinase, Platelet Type; 6

  • AA Sequence

    MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDDQIPKTNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV

  • Predicted Molecular Mass

    113.4 kDa

  • Molecular Weight

    Approximately 113 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% glycerol, pH 8.0, 0.5 mM TCEP, 22.5% trehalose .
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 1 year from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • PFKP Protein, Human (sf9, His-GST) Product Image
    1/16
  • PFKP Protein, Human (sf9, His-GST) Product Image