PRPF4B Protein, Human (sf9, GST)

PRPF4B Protein, Human (sf9, GST) is the recombinant human-derived PRPF4B, expressed by Sf9 insect cells , with GST labeled tag. ,

PRPF4B is a serine/threonine kinase involved in spliceosomal assembly, mitosis, and signaling regulation. It bridges chromatin-mediated transcriptional regulation with pre-mRNA splicing, interacting with and phosphorylating U4/U6-U5 tri-snRNP components PRPF6 and PRPF31 to facilitate spliceosome B complex formation. PRPF4B also regulates transcription and the spindle assembly checkpoint (SAC), associating with U5 snRNP and NCOR1 deacetylase complexes to coordinate splicing with chromatin remodeling. It likely phosphorylates SMARCA4 and NCOR1, as well as SRSF1. During mitosis, PRPF4B localizes to kinetochores and is essential for recruiting/maintaining checkpoint proteins (e.g., MAD1L1, MAD2L1). Additionally, it phosphorylates transcription factors ELK1, KLF13, and nuclear YAP1/WWTR1(TAZ), the latter triggering nuclear exclusion and modulating the Hippo pathway (implicated in tissue growth control).

Human

Sf9 insect cells

N-GST

Q13523 (K551-I1007)

8899

Partial

KYKYLAEDSNMSVPSEPSSPQSSTRTRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKLLAPDMFTESDDMFAAYFDSARLRAAGIGKDFKENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYGYTGQGVFSNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPYLVSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFIQEKI

Approximately 79 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of PBS, pH 7.5, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.