PTP alpha/PTPRA Protein, Human (sf9, His-GST)

PTP alpha/PTPRA Protein, a tyrosine protein phosphatase, participates in integrin-mediated focal adhesion formation. Upon integrin engagement, it recruits BCAR3, BCAR1, and CRK to focal adhesions, promoting SRC-mediated phosphorylation of BRAC1. This leads to the activation of PAK and small GTPases RAC1 and CDC42. PTP alpha/PTPRA Protein, Human (sf9, His-GST) is the recombinant human-derived PTP alpha/PTPRA protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

PTP alpha/PTPRA, a tyrosine protein phosphatase, plays a pivotal role in integrin-mediated focal adhesion formation. Upon integrin engagement, it selectively recruits BCAR3, BCAR1, and CRK to focal adhesions, fostering SRC-mediated phosphorylation of BRAC1. This phosphorylation event activates downstream signaling cascades involving PAK, as well as small GTPases RAC1 and CDC42. Through its participation in these intricate molecular processes, PTP alpha/PTPRA contributes to the dynamic regulation of focal adhesions, influencing cellular responses to integrin signaling.

Human

Sf9 insect cells

N-His;N-GST

P18433-2 (A174-K793)

5786  [NCBI]

Partial

AGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYPPLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPYDHSRVHLTPVEGVPDSDYINASFINGYQEKNKFIAAQGPKEETVNDFWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDVTVLVDYTVRKFCIQQVGDMTNRKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKACNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMHTERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALLEHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK

Approximately 90 kDa,based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.