PTP alpha/PTPRA Protein, Human (sf9, His-GST)
PTP alpha/PTPRA Protein, a tyrosine protein phosphatase, participates in integrin-mediated focal adhesion formation. Upon integrin engagement, it recruits BCAR3, BCAR1, and CRK to focal adhesions, promoting SRC-mediated phosphorylation of BRAC1. This leads to the activation of PAK and small GTPases RAC1 and CDC42. PTP alpha/PTPRA Protein, Human (sf9, His-GST) is the recombinant human-derived PTP alpha/PTPRA protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
PTP alpha/PTPRA Protein, a tyrosine protein phosphatase, participates in integrin-mediated focal adhesion formation. Upon integrin engagement, it recruits BCAR3, BCAR1, and CRK to focal adhesions, promoting SRC-mediated phosphorylation of BRAC1. This leads to the activation of PAK and small GTPases RAC1 and CDC42. PTP alpha/PTPRA Protein, Human (sf9, His-GST) is the recombinant human-derived PTP alpha/PTPRA protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
PTP alpha/PTPRA, a tyrosine protein phosphatase, plays a pivotal role in integrin-mediated focal adhesion formation. Upon integrin engagement, it selectively recruits BCAR3, BCAR1, and CRK to focal adhesions, fostering SRC-mediated phosphorylation of BRAC1. This phosphorylation event activates downstream signaling cascades involving PAK, as well as small GTPases RAC1 and CDC42. Through its participation in these intricate molecular processes, PTP alpha/PTPRA contributes to the dynamic regulation of focal adhesions, influencing cellular responses to integrin signaling.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
P18433-2 (A174-K793)
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Molecular Construction
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N-term
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His-GST
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PTPRA (A174-K793)
Accession # P18433-2 -
C-term
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Protein Length
Partial
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Synonyms
PTPRA; Protein-Tyrosine Phosphatase Alpha; Prev. PTPRL2; Leukocyte Common Antigen-Related Peptide (Protein Tyrosine Phosphate); Prev. PTPA; Protein Tyrosine Phosphatase, Receptor Type, Alpha Polypeptide; Receptor-Type Tyrosine-Protein Phosphatase Alpha; P
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AA Sequence
AGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYPPLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPYDHSRVHLTPVEGVPDSDYINASFINGYQEKNKFIAAQGPKEETVNDFWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDVTVLVDYTVRKFCIQQVGDMTNRKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKACNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMHTERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALLEHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK
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Predicted Molecular Mass
99 kDa
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Molecular Weight
Approximately 90 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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