PTPN12 Protein, Human (sf9, His-GST)

The PTPN12 protein is a multifunctional enzyme that can dephosphorylate a variety of proteins and regulate cell signaling cascades. According to literature, PTPN12 selectively targets tyrosine kinases such as ERBB2 and PTK2B/PYK2 to control related pathways. PTPN12 Protein, Human (sf9, His-GST) is the recombinant human-derived PTPN12 protein, expressed by Sf9 insect cells , with N-GST, N-His labeled tag.

PTPN12 Protein, the subject, functions as a versatile enzyme capable of dephosphorylating a range of proteins, thereby playing a crucial role in the regulation of various cellular signaling cascades, as documented in the literature. Among its substrates, PTPN12 selectively dephosphorylates cellular tyrosine kinases, including ERBB2 and PTK2B/PYK2, exerting regulatory control over signaling pathways involving these kinases. The specificity of PTPN12 is highlighted by its ability to dephosphorylate ERBB2 at specific tyrosine residues, such as 'Tyr-1112,' 'Tyr-1196,' and/or 'Tyr-1248.' The intricate dephosphorylation activity of PTPN12 underscores its significance in modulating key signaling events and fine-tuning cellular responses mediated by tyrosine kinase pathways.

Human

Sf9 insect cells

N-GST;N-His

Q05209 (M1-Q355)

5782  [NCBI]

Partial

MEQVEILRKFIQRVQAMKSPDHNGEDNFARDFMRLRRLSTKYRTEKIYPTATGEKEENVKKNRYKDILPFDHSRVKLTLKTPSQDSDYINANFIKGVYGPKAYVATQGPLANTVIDFWRMIWEYNVVIIVMACREFEMGRKKCERYWPLYGEDPITFAPFKISCEDEQARTDYFIRTLLLEFQNESRRLYQFHYVNWPDHDVPSSFDSILDMISLMRKYQEHEDVPICIHCSAGCGRTGAICAIDYTWNLLKAGKIPEEFNVFNLIQEMRTQRHSAVQTKEQYELVHRAIAQLFEKQLQLYEIHGAQKIADGVNEINTENMVSSIEPEKQDSPPPKPPRTRSCLVEGDAKEEILQ

Approximately 64 kDa,based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.