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  5. Semaphorin-4D/SEMA4D Protein, Human (HEK293, hFc)

Semaphorin-4D/SEMA4D Protein, Human (HEK293, hFc)

Cat. No.: HY-P74565
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Semaphorin-4D/SEMA4D protein, a receptor for PLXNB1 and PLXNB2, regulates cell-cell signaling. It promotes inhibitory synapse development via PLXNB1, modulates neurite arborization through RHOA activation, and enhances viability of B-cells. SEMA4D induces endothelial cell migration via PTK2B/PYK2, SRC, and PI3K-AKT pathway. It forms homodimers and interacts with PLXNB2 and PLXNB1. Semaphorin-4D/SEMA4D Protein, Human (HEK293, hFc) is the recombinant human-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Semaphorin-4D/SEMA4D Protein, Human (HEK293, hFc) is 734 a.a., with molecular weight of ~105.95 kDa.

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Description

Semaphorin-4D/SEMA4D protein, a receptor for PLXNB1 and PLXNB2, regulates cell-cell signaling. It promotes inhibitory synapse development via PLXNB1, modulates neurite arborization through RHOA activation, and enhances viability of B-cells. SEMA4D induces endothelial cell migration via PTK2B/PYK2, SRC, and PI3K-AKT pathway. It forms homodimers and interacts with PLXNB2 and PLXNB1. Semaphorin-4D/SEMA4D Protein, Human (HEK293, hFc) is the recombinant human-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Semaphorin-4D/SEMA4D Protein, Human (HEK293, hFc) is 734 a.a., with molecular weight of ~105.95 kDa.

Background

Semaphorin-4D/SEMA4D Protein, a cell surface receptor for PLXNB1 and PLXNB2, plays a crucial role in cell-cell signaling. It is involved in regulating GABAergic synapse development and promotes the development of inhibitory synapses in a PLXNB1-dependent manner. By activating PLXNB1 and interacting with it, SEMA4D modulates the complexity and arborization of developing neurites in hippocampal neurons, mediating the activation of RHOA. Additionally, SEMA4D promotes the migration of cerebellar granule cells and induces B-cells to aggregate, enhancing their viability in vitro. In the immune system, SEMA4D plays a role in inducing endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. It forms homodimers and interacts with PLXNB2, as well as interacts with PLXNB1.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q92854 (M1-R734)

Gene ID
Molecular Construction
N-term
SEMA4D (M1-R734)
Accession # Q92854
hFc
C-term
Synonyms
SEMA4D; Semaphorin-4D; M-Sema G; Semaphorin-C-like 2; Sema J; CD100; Semacl2
Molecular Weight

Approximately 105.95 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Semaphorin-4D/SEMA4D Protein, Human (HEK293, hFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Semaphorin-4D/SEMA4D Protein, Human (HEK293, hFc)
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HY-P74565
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