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Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His, solution)

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Based on 1 Customer Validation

Semaphorin-7A/SEMA7A proteins critically regulate integrin-mediated signaling, affecting cell migration and immune responses.It promotes focal adhesion complex formation, activates PTK2/FAK1, and leads to the phosphorylation of MAPK1 and MAPK3.Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived Semaphorin-7A/SEMA7A protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His, solution)
  • Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His, solution) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Semaphorin-7A/SEMA7A proteins critically regulate integrin-mediated signaling, affecting cell migration and immune responses.It promotes focal adhesion complex formation, activates PTK2/FAK1, and leads to the phosphorylation of MAPK1 and MAPK3.Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived Semaphorin-7A/SEMA7A protein, expressed by HEK293 , with C-His labeled tag.

Background

The Semaphorin-7A/SEMA7A protein plays a crucial role in integrin-mediated signaling, contributing to the regulation of cell migration and immune responses. It facilitates the formation of focal adhesion complexes and activates the protein kinase PTK2/FAK1, leading to the subsequent phosphorylation of MAPK1 and MAPK3. Moreover, it enhances the production of pro-inflammatory cytokines by monocytes and macrophages. This protein also plays a significant role in modulating inflammation and T-cell-mediated immune responses. Additionally, it promotes axon growth in the embryonic olfactory bulb and aids in the attachment, spreading, and dendrite outgrowth in melanocytes. It interacts with PLXNC1 and the integrins ITGA1 and ITGB1.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    Q9QUR8 (Q45-A646)

  • Gene ID
    20361  [NCBI]
  • Molecular Construction
    • N-term
    • SEMA7A (Q45-A646)
      Accession # Q9QUR8
    • 8*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SEMA7A; CDw108; Prev. SEMAL; Sema Domain, Immunoglobulin Domain (Ig), And GPI Membrane Anchor, (Semaphorin) 7A; CD108; Sema Domain, Immunoglobulin Domain (Ig), And GPI Membrane Anchor, 7A; Semaphorin-7A; Semaphorin 7A (John Milton Hagen Blood Group); Sema

  • AA Sequence

    QGHSRSGPRISAVWKGQDHVDFSQPEPHTVLFHEPGSFSVWVGGRGKVYHFNFPEGKNASVRTVNIGSTKGSCQDKQDCGNYITLLERRGNGLLVCGTNARKPSCWNLVNDSVVMSLGEMKGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNRNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDRVFRTSSLKGYHMGLPNPRPGMCLPKKQPIPTETFQVADSHPEVAQRVEPMGPLKTPLFHSKYHYQKVVVHRMQASNGETFHVLYLTTDRGTIHKVVESGDQDHSFVFNIMEIQPFHRAAAIQAISLDADRRKLYVTSQWEVSQVPLDMCEVYSGGCHGCLMSRDPYCGWDQDRCVSIYSSQRSVLQSINPAEPHRECPNPKPDEAPLQKVSLARNSRYYLTCPMESRHATYLWRHEENVEQSCEPGHQSPSCILFIENLTARQYGHYRCEAQEGSYLREAQHWELLPEDRALAEQLMGHARALA

  • Predicted Molecular Mass

    69.5 kDa

  • Molecular Weight

    Approximately 70-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His, solution) Product Image
    1/16
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His, solution)
  • Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, His, solution) Product Image