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Serpin A3N Protein, Rat (HEK293, His)

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The Serpin A3N Protein, belonging to the serpin family, is predominantly expressed in the liver, where it regulates protease activity.As a serine protease inhibitor, Serpin A3N plays a crucial role in modulating enzymatic processes within the liver, highlighting its significance in hepatic functions and maintaining the balance of proteolytic activities in this vital organ.Serpin A3N Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin A3N protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The Serpin A3N Protein, belonging to the serpin family, is predominantly expressed in the liver, where it regulates protease activity.As a serine protease inhibitor, Serpin A3N plays a crucial role in modulating enzymatic processes within the liver, highlighting its significance in hepatic functions and maintaining the balance of proteolytic activities in this vital organ.Serpin A3N Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin A3N protein, expressed by HEK293 , with C-His labeled tag.

Background

The Serpin A3N Protein primarily localizes in the liver. This serine protease inhibitor, belonging to the serpin family, is notably expressed in hepatic tissues. In the liver, Serpin A3N is involved in the regulation of protease activity, acting as an inhibitor to modulate enzymatic processes. Its specific localization in the liver underscores its significance in hepatic functions and emphasizes its role in maintaining the balance of proteolytic activities within this vital organ.

Verified Bioactivity

Measured by its ability to inhibit Granzyme B cleavage of tert-butoxycarbonyl-Ala-Ala-Asp-thiobenzyl ester (Boc-AAD-SBzl) that incubate at room temperature in kinetic mode for 5 minutes. The IC50 is 65.38 nM. (Activation description: The proenzyme needs to be activated by Cathepsin C, Mouse (HY-P704018) an activated form.)

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    P09006 (T30-K418)

  • Gene ID
    24795  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin A3N (T30-K418)
      Accession # P09006
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    Serine protease inhibitor A3N; Serpin A3N; Serpina3n; Spi2

  • AA Sequence

    TLFHEDQDKGTQLDSLTLASINTDFAFSLYKKLALRNPDKNVVFSPLSISAALAVVSLGAKGNSMEEILEGLKFNLTETPETEIHRGFGHLLQRLSQPRDEIQISTGNALFIEKRLQVLAEFQEKAKALYQAEAFTADFQQSREAKKLINDYVSKQTQGKIQGLITNLAKKTSMVLVNYIYFKGKWKVPFDPRDTFQSEFYSGKRRPVKVPMMKLEDLTTPYVRDEELNCTVVELKYTGNASALFILPDQGKMQQVEASLQPETLRRWKDSLRPSMIDELYLPKFSISADYNLEDVLPELGIKEVFSTQADLSGITGDKDLMVSQVVHKAVLDVAETGTEAAAATGVKFVPMSAKLDPLIIAFDRPFLMIISDTETAIAPFLAKIFNPK

  • Predicted Molecular Mass

    44.7 kDa

  • Molecular Weight

    Approximately 50-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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