SNCA Protein, Mouse

SNCA proteins are critical for synaptic activity and regulate vesicle trafficking and neurotransmitter release. As a monomer, it enhances the vesicle exocytosis process. SNCA Protein, Mouse is the recombinant mouse-derived SNCA protein, expressed by E. coli , with tag free.

SNCA protein, a pivotal player in synaptic activity, performs diverse roles in the regulation of synaptic vesicle trafficking and neurotransmitter release. It functions as a monomer in synaptic vesicle exocytosis, enhancing processes such as vesicle priming, fusion, and dilation of exocytotic fusion pores. Additionally, SNCA acts as a molecular chaperone when in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane. This chaperone activity is vital for maintaining normal SNARE-complex assembly during aging. SNCA also contributes to the regulation of dopamine neurotransmission by interacting with the dopamine transporter (DAT1) and modulating its activity. Existing as both a soluble monomer and homotetramer, SNCA dynamically balances tetramers and monomers, with the tetramer playing a crucial role in homeostasis. SNCA engages in various interactions with proteins such as UCHL1, synphilin-1/SNCAIP, CALM1, STXBP1, VAMP2, SNAP25, RPH3A, RAB3A, SERF1A, and SEPTIN4, illustrating its involvement in complex molecular networks governing synaptic function and integrity.

Immobilized Mouse Alpha-Synuclein at 1 μg/mL (100 μL/well) can bind Anti-Synucelin alpha/beta Monoclonal Antibody, the ED₅₀ for this effect is 316.4 ng/mL.

Mouse

E. coli

Tag Free

O55042-1 (M1-A140)

20617  [NCBI]

Full Length of Isoform-1

MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA

Approximately 17 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.