SR-BI/SCARB1 Protein, Mouse (HEK293, His)

The SR-BI/SCARB1 protein is a multifunctional receptor that binds a variety of ligands, including phospholipids, cholesteryl esters, lipoproteins, phosphatidylserine, and apoptotic cells. Both isoform 1 and isoform 2 act as high-density lipoprotein (HDL) receptors, promoting cholesterol ether uptake and HDL-dependent cholesterol efflux. SR-BI/SCARB1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived SR-BI/SCARB1 protein, expressed by HEK293 , with C-His labeled tag.

SR-BI/SCARB1 functions as a versatile receptor with binding capabilities for various ligands, including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and apoptotic cells. Both isoform 1 and isoform 2 serve as receptors for high-density lipoprotein (HDL), mediating the selective uptake of cholesteryl ether and facilitating HDL-dependent cholesterol efflux. Additionally, SR-BI/SCARB1 plays a role in the transport of free and esterified cholesterol between the cell surface and apolipoprotein B (apoB)-containing lipoproteins, as well as modified lipoproteins, albeit less efficiently than HDL. The receptor may also participate in the phagocytosis of apoptotic cells through its phosphatidylserine binding activity. Notably, the C-terminal region of SR-BI/SCARB1 interacts with PDZK1, highlighting its potential involvement in complex cellular processes and signaling pathways.

Mouse

HEK293

C-His

Q61009/NP_058021.1 (P33-V440)

20778  [NCBI]

Extracellular Domain

PSLIKQQVLKNVRIDPSSLSFGMWKEIPVPFYLSVYFFEVVNPNEVLNGQKPVVRERGPYVYREFRQKVNITFNDNDTVSFVENRSLHFQPDKSHGSESDYIVLPNILVLGGSILMESKPVSLKLMMTLALVTMGQRAFMNRTVGEILWGYDDPFVHFLNTYLPDMLPIKGKFGLFVGMNNSNSGVFTVFTGVQNFSRIHLVDKWNGLSKIDYWHSEQCNMINGTSGQMWAPFMTPESSLEFFSPEACRSMKLTYNESRVFEGIPTYRFTAPDTLFANGSVYPPNEGFCPCRESGIQNVSTCRFGAPLFLSHPHFYNADPVLSEAVLGLNPNPKEHSLFLDIHPVTGIPMNCSVKMQLSLYIKSVKGIGQTGKIEPVVLPLLWFEQSGAMGGKPLSTFYTQLVLMPQV

Approximately 70-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.