STXBP3 Protein, Human (sf9, His-GST)
STXBP3 protein, together with STX4 and VAMP2, may promote insulin-dependent GLUT4 movement and fusion of GLUT4-containing vesicles with the adipocyte cell surface. It interacts directly with DOC2B on the cell membrane (excluding STX4 interaction) and regulates glucose-stimulated insulin secretion. STXBP3 Protein, Human (sf9, His-GST) is the recombinant human-derived STXBP3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
STXBP3 protein, together with STX4 and VAMP2, may promote insulin-dependent GLUT4 movement and fusion of GLUT4-containing vesicles with the adipocyte cell surface. It interacts directly with DOC2B on the cell membrane (excluding STX4 interaction) and regulates glucose-stimulated insulin secretion. STXBP3 Protein, Human (sf9, His-GST) is the recombinant human-derived STXBP3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
The STXBP3 protein, in conjunction with STX4 and VAMP2, potentially functions in the insulin-dependent movement of GLUT4 and the docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. Additionally, STXBP3 interacts with DOC2B directly at the cell membrane, excluding interaction with STX4, thereby regulating glucose-stimulated insulin secretion. Furthermore, STXBP3 engages in interaction with STX4, indicating its involvement in diverse cellular processes, particularly those related to vesicle trafficking and insulin-responsive glucose transport in adipocytes. These molecular associations underscore the multifaceted role of STXBP3 in cellular dynamics and insulin-regulated pathways.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
O00186 (M1-E592)
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Molecular Construction
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N-term
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His-GST
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STXBP3 (M1-E592)
Accession # O00186 -
C-term
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Protein Length
Full Length
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Synonyms
STXBP3; Unc18-3; Syntaxin Binding Protein 3; PSP; UNC-18C; Syntaxin 4 Binding Protein; Syntaxin-Binding Protein 3; MUNC18-3; Protein Unc-18 Homolog 3; MUNC18C; Protein Unc-18 Homolog C; Unc-18C; Platelet Sec1 Protein
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AA Sequence
MAPPVAERGLKSVVWQKIKATVFDDCKKEGEWKIMLLDEFTTKLLASCCKMTDLLEEGITVVENIYKNREPVRQMKALYFITPTSKSVDCFLHDFASKSENKYKAAYIYFTDFCPDNLFNKIKASCSKSIRRCKEINISFIPHESQVYTLDVPDAFYYCYSPDPGNAKGKDAIMETMADQIVTVCATLDENPGVRYKSKPLDNASKLAQLVEKKLEDYYKIDEKSLIKGKTHSQLLIIDRGFDPVSTVLHELTFQAMAYDLLPIENDTYKYKTDGKEKEAILEEEDDLWVRIRHRHIAVVLEEIPKLMKEISSTKKATEGKTSLSALTQLMKKMPHFRKQITKQVVHLNLAEDCMNKFKLNIEKLCKTEQDLALGTDAEGQKVKDSMRVLLPVLLNKNHDNCDKIRAILLYIFSINGTTEENLDRLIQNVKIENESDMIRNWSYLGVPIVPQSQQGKPLRKDRSAEETFQLSRWTPFIKDIMEDAIDNRLDSKEWPYCSQCPAVWNGSGAVSARQKPRANYLEDRKNGSKLIVFVIGGITYSEVRCAYEVSQAHKSCEVIIGSTHVLTPKKLLDDIKMLNKPKDKVSLIKDE
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Predicted Molecular Mass
95.6 kDa
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Molecular Weight
Approximately 96 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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