Tau/MAPT Protein, Rat (HEK293, His)

Microtubule-associated protein tau (MAPT) critically promotes microtubule assembly and stability, influencing neuronal polarity by acting as a vital linker protein. Its dual role involves the C-terminus binding axonal microtubules and the N-terminus engaging neural plasma membrane components. Tau's cellular localization, especially near the centrosome, determines axonal polarity. Short tau isoforms offer cytoskeletal plasticity, while longer isoforms likely stabilize microtubules. MAPT engages with various proteins, including MARK1, MARK2, SQSTM1, LRP1, showcasing its multifaceted role in cellular processes. The interaction with LRP1, leading to endocytosis, highlights the intricate regulatory mechanisms governing tau's functional versatility. Tau/MAPT Protein, Rat (HEK293, His) is the recombinant rat-derived Tau/MAPT protein, expressed by HEK293, with N-10*His labeled tag. The total length of Tau/MAPT Protein, Rat (HEK293, His) is 751 a.a., with molecular weight of 81.2 kDa.

Microtubule-associated protein tau (MAPT) plays a pivotal role in cellular dynamics by promoting microtubule assembly and stability, potentially contributing to the establishment and maintenance of neuronal polarity. Its functional architecture reveals a dual role, with the C-terminus binding axonal microtubules and the N-terminus engaging neural plasma membrane components, implying that tau acts as a crucial linker protein between the two structures. The predetermined axonal polarity is governed by tau's localization within the neuronal cell, specifically in the domain defined by the centrosome. Notably, the short isoforms of tau confer plasticity to the cytoskeleton, while the longer isoforms may preferentially contribute to its stabilization. MAPT engages in a complex network of interactions with various proteins, including MARK1, MARK2, MARK3, SQSTM1, PSMC2, FKBP4, CSNK1D, SGK1, EPM2A, PIN1, LRRK2, and LRP1, showcasing its multifaceted involvement in cellular processes. Particularly, its interaction with LRP1, leading to endocytosis, underscores the intricate regulatory mechanisms governing tau's functional versatility.

Rat

HEK293

N-10*His

P19332 (A2-L752)

29477  [NCBI]

AEPRQEFDTMEDQAGDYTMLQDQEGDMDHGLKESPPQPPADDGSEEPGSETSDAKSTPTAEDVTAPLVEERAPDKQATAQSHTEIPEGTTAEEAGIGDTPNMEDQAAGHVTQEPQKVEIFSQSLLVEPGRREGQAPDSGISDWTHQQVPSMSGAPLPPQGLREATHQPLGTRPEDVERSHPASELLWQESPQKEAWGKDRLGSEEEVDEDITMDESSQESPPSQASLAPGTATPQARSVSASGVSGETTSIPGFPAEGSIPLPADFFSKVSAETQASPPEGPGTGPSEEGHEAAPEFTFHVEIKASAPKEQDLEGATVVGAPAEEQKARGPSVGKGTKEASLLEPTDKQPAAGLPGRPVSRVPQLKARVAGVSKDRTGNDEKKAKTSTPSCAKTPSNRPCLSPTRPTPGSSDPLIKPSSPAVCPEPATSPKYVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAATPGQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVHIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

81.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.