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TRIB3 Protein, Human (sf9, GST)

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Based on 1 publication(s) in Google Scholar

TRIB3 protein is an inactive kinase that centrally regulates the integrated stress response (ISR) and inhibits DDIT3/CHOP transcriptional activity. It causes DDIT3/CHOP-dependent cell death during endoplasmic reticulum (ER) stress, particularly in programmed neuronal cell death. TRIB3 Protein, Human (sf9, GST) is the recombinant human-derived TRIB3 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

TRIB3 protein is an inactive kinase that centrally regulates the integrated stress response (ISR) and inhibits DDIT3/CHOP transcriptional activity. It causes DDIT3/CHOP-dependent cell death during endoplasmic reticulum (ER) stress, particularly in programmed neuronal cell death. TRIB3 Protein, Human (sf9, GST) is the recombinant human-derived TRIB3 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

Background

The TRIB3 protein, an inactive kinase, operates as a pivotal regulator of the integrated stress response (ISR), a mechanism crucial for adapting to diverse stress conditions. It functions as an inhibitor of the transcriptional activity of DDIT3/CHOP and plays a role in DDIT3/CHOP-dependent cell death during endoplasmic reticulum (ER) stress, particularly in the context of programmed neuronal cell death. Acting as a negative feedback regulator within the ISR, TRIB3 interacts with ATF4, inhibiting ATF4 transcriptional activity while being promoted by ATF4 expression. Moreover, TRIB3 disrupts insulin signaling by directly binding to Akt kinases, impeding their activation, and may mask the 'Thr-308' phosphorylation site in AKT1. Additionally, TRIB3 interacts with various signaling molecules, including NF-kappa-B transactivator p65 RELA, MAPK kinases, and APOBEC3 proteins, showcasing its multifaceted role in diverse cellular processes and stress responses.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-GST
  • Accession

    Q96RU7 (M1-G358)

  • Gene ID
    57761  [NCBI]
  • Molecular Construction
    • N-term
    • GST
    • TRIB3 (M1-G358)
      Accession # Q96RU7
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    TRIB3; C20orf97; NIPK; SKIP3; TRB3; Tribbles homolog 3; TRB-3; Neuronal cell death-inducible putative kinase; SINK; p65-interacting inhibitor of NF-kappa-B; Neuronal Cell Death Inducible Putative Kinase; Chromosome 20 Open Reading Frame 97; P65-Interactin

  • AA Sequence

    MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYQALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG

  • Predicted Molecular Mass

    66.1 kDa

  • Molecular Weight

    Approximately 66 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM Reduced Glutathione, 10% glycerol, 0.5 mM PMSF.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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