1. Recombinant Proteins
  2. Ubiquitin Related Proteins
  3. Deubiquitinase
  4. Ubiquitin-Specific Protease
  5. Ubiquitin-Specific Protease 7
  6. USP7 Protein, Human (sf9)

USP7 Protein, Human (sf9)

Cat. No.: HY-P74478
Handling Instructions

The USP7 protein is a hydrolase that deubiquitinates multiple targets, including FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5, and DAXX. It cooperates with DAXX to enhance the E3 ligase activity of MDM2 and promote ubiquitination and degradation of p53/TP53. USP7 Protein, Human (sf9) is the recombinant human-derived USP7 protein, expressed by Sf9 insect cells , with tag free. The total length of USP7 Protein, Human (sf9) is 353 a.a., with molecular weight of ~41 kDa.

For research use only. We do not sell to patients.

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Description

The USP7 protein is a hydrolase that deubiquitinates multiple targets, including FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5, and DAXX. It cooperates with DAXX to enhance the E3 ligase activity of MDM2 and promote ubiquitination and degradation of p53/TP53. USP7 Protein, Human (sf9) is the recombinant human-derived USP7 protein, expressed by Sf9 insect cells , with tag free. The total length of USP7 Protein, Human (sf9) is 353 a.a., with molecular weight of ~41 kDa.

Background

The USP7 protein, functioning as a hydrolase, plays a pivotal role in deubiquitinating various target proteins, including FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5, and DAXX. Collaborating with DAXX, it prevents MDM2 self-ubiquitination and enhances MDM2's E3 ligase activity toward p53/TP53, promoting p53/TP53 ubiquitination and subsequent degradation. Furthermore, USP7 is involved in cell proliferation during early embryonic development, transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage, and maintenance of DNA methylation. It regulates chromatin structure, exhibits a preference for 'Lys-48'-linked ubiquitin chains, and modulates the circadian clock. Additionally, USP7 contributes to the stabilization of specific proteins involved in gluconeogenesis, neural progenitor cell maintenance, and actin polymerization at endosomal surfaces. Importantly, its diverse functions highlight its significance in cellular processes and response to microbial infections, such as its involvement in the stabilization and trans-activation of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.

Species

Human

Source

Sf9 insect cells

Tag

Tag Free

Accession

Q93009 (K208-E560)

Gene ID
Molecular Construction
N-term
USP7 (K208-E560)
Accession # Q93009
C-term
Synonyms
Ubiquitin carboxyl-terminal hydrolase 7; USP7; HAUSP
Molecular Weight

Approximately 41 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
USP7 Protein, Human (sf9)
Cat. No.:
HY-P74478
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