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VE-Cadherin Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

VE-cadherin is critical for cellular functions such as β-catenin binding and signaling receptor activity, and is involved in processes such as cell-cell junction organization and vascular maturation. It is predicted to localize in cell-cell junctions and nuclei, serving as a biomarker for pulmonary hypertension. VE-Cadherin Protein, Rat (HEK293, His) is the recombinant rat-derived VE-Cadherin protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for VE-Cadherin Protein, Rat (HEK293, His)
  • Experimental Validation Results for VE-Cadherin Protein, Rat (HEK293, His)
  • VE-Cadherin Protein, Rat (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

VE-cadherin is critical for cellular functions such as β-catenin binding and signaling receptor activity, and is involved in processes such as cell-cell junction organization and vascular maturation. It is predicted to localize in cell-cell junctions and nuclei, serving as a biomarker for pulmonary hypertension. VE-Cadherin Protein, Rat (HEK293, His) is the recombinant rat-derived VE-Cadherin protein, expressed by HEK293 , with C-His labeled tag.

Background

VE-Cadherin Protein is predicted to play a crucial role in various cellular functions, including beta-catenin binding, enzyme binding, and signaling receptor binding activities. It is anticipated to be involved in processes such as cell-cell junction organization, regulation of protein modification, and regulation of protein-containing complex assembly. VE-Cadherin is predicted to function upstream of blood vessel maturation, cell-cell adhesion, and negative regulation of cell population proliferation. The protein is expected to be located in cell-cell junctions, the external side of the plasma membrane, and the nucleus, and it may participate in catenin complex formation. With predicted activity in adherens junctions and bicellular tight junctions, VE-Cadherin serves as a biomarker for pulmonary hypertension. Its human ortholog, cadherin 5 (CDH5), shows biased expression in lung and heart tissues, highlighting its importance in cardiovascular and pulmonary contexts.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED50 for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×104 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED50 for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×104units/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    NP_001100877 (G25-Q585)

  • Gene ID
    307618  [NCBI]
  • Molecular Construction
    • N-term
    • VE-Cadherin (G25-Q585)
      Accession # NP_001100877
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CDH5; Cadherin 5, Type 2, VE-Cadherin (Vascular Epithelium); Cadherin 5; Cadherin 5, Type 2 (Vascular Endothelium); Vascular Endothelial Cadherin; 7B4 Antigen; VE-Cadherin; CDNA FLJ51088, Highly Similar To Cadherin-5; Cadherin-5; Endothelial-Specific Cadh

  • AA Sequence

    GPNMLPVHQRQKRDWIWNQMHIDEEKNESLPHYVGKIKSNVNRQNAKYVLQGEQAGKIFRVDADTGDVLAYERLDREKVSEYFLTALIVDKKTNKNLEQPSSFTVKVHDVNDNWPVFTHQVFNASVPEMSAIGTSVIRVTATDADDPTVAGHATVLYQMVKGNEYFAIDNSGLIFTKDKNLDRETRAEYKIVVEAQDAQGLRGESGTATVLIRLEDINDNFPIFTQSTYTFSVPEDIRVGKPLGSLSVEDPDEPQNRMTKYSIMQGEYRDTFTIETDPDRNEGIIKPTKPLDYELIQQYTFHIEATDPTIRLGYLSSTAGKNKAKIIINVLDVDEPPVFQRRFYHFHLPENKKKPLIGTVVAKDPDKAQRSIGYSIRKTSDRGQYFRITKQGNIYNEKELDRETHAWYNLTVEANELDSRGNPVGKESIVQIYIEVLDENDNAPEFAQPYEPKVCENAAQGKLVVQISATDKDVVPVNTKFKFALKNEDSNFTLINNHDNTANITVKYGQFNREHTKFHYLPVLISDNGMPSLTGTGTLTVAVCKCNEQGEFTFCEEMAAQ

  • Molecular Weight

    Approximately 91 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED50 for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×104units/mg.

  • VE-Cadherin Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for VE-Cadherin Protein, Rat (HEK293, His)
  • Experimental Validation Results for VE-Cadherin Protein, Rat (HEK293, His)
  • VE-Cadherin Protein, Rat (HEK293, His) Product Image