VE-Cadherin Protein, Rat (HEK293, His)

VE-cadherin is critical for cellular functions such as β-catenin binding and signaling receptor activity, and is involved in processes such as cell-cell junction organization and vascular maturation. It is predicted to localize in cell-cell junctions and nuclei, serving as a biomarker for pulmonary hypertension. VE-Cadherin Protein, Rat (HEK293, His) is the recombinant rat-derived VE-Cadherin protein, expressed by HEK293 , with C-His labeled tag. The total length of VE-Cadherin Protein, Rat (HEK293, His) is 561 a.a., with molecular weight of ~80-95 kDa.

VE-Cadherin Protein is predicted to play a crucial role in various cellular functions, including beta-catenin binding, enzyme binding, and signaling receptor binding activities. It is anticipated to be involved in processes such as cell-cell junction organization, regulation of protein modification, and regulation of protein-containing complex assembly. VE-Cadherin is predicted to function upstream of blood vessel maturation, cell-cell adhesion, and negative regulation of cell population proliferation. The protein is expected to be located in cell-cell junctions, the external side of the plasma membrane, and the nucleus, and it may participate in catenin complex formation. With predicted activity in adherens junctions and bicellular tight junctions, VE-Cadherin serves as a biomarker for pulmonary hypertension. Its human ortholog, cadherin 5 (CDH5), shows biased expression in lung and heart tissues, highlighting its importance in cardiovascular and pulmonary contexts.

Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED₅₀ for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×10⁴ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED₅₀ for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×10⁴units/mg.

Rat

HEK293

C-His

NP_001100877 (G25-Q585)

307618  [NCBI]

GPNMLPVHQRQKRDWIWNQMHIDEEKNESLPHYVGKIKSNVNRQNAKYVLQGEQAGKIFRVDADTGDVLAYERLDREKVSEYFLTALIVDKKTNKNLEQPSSFTVKVHDVNDNWPVFTHQVFNASVPEMSAIGTSVIRVTATDADDPTVAGHATVLYQMVKGNEYFAIDNSGLIFTKDKNLDRETRAEYKIVVEAQDAQGLRGESGTATVLIRLEDINDNFPIFTQSTYTFSVPEDIRVGKPLGSLSVEDPDEPQNRMTKYSIMQGEYRDTFTIETDPDRNEGIIKPTKPLDYELIQQYTFHIEATDPTIRLGYLSSTAGKNKAKIIINVLDVDEPPVFQRRFYHFHLPENKKKPLIGTVVAKDPDKAQRSIGYSIRKTSDRGQYFRITKQGNIYNEKELDRETHAWYNLTVEANELDSRGNPVGKESIVQIYIEVLDENDNAPEFAQPYEPKVCENAAQGKLVVQISATDKDVVPVNTKFKFALKNEDSNFTLINNHDNTANITVKYGQFNREHTKFHYLPVLISDNGMPSLTGTGTLTVAVCKCNEQGEFTFCEEMAAQ

Approximately 80-95 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.