VEGFR-3/FLT4 Protein, Mouse (HEK293, mFc)
Based on 1 Customer Validation
VEGFR-3/FLT4 proteins are tyrosine protein kinase receptors for VEGFC and VEGFD and are critical in adult lymphangiogenesis and embryonic vascular development. It promotes endothelial cell function, angiogenesis, and forms a positive feedback loop that increases VEGFC production. VEGFR-3/FLT4 Protein, Mouse (HEK293, mFc) is the recombinant mouse-derived VEGFR-3/FLT4 protein, expressed by HEK293, with C-mFc labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
VEGFR-3/FLT4 proteins are tyrosine protein kinase receptors for VEGFC and VEGFD and are critical in adult lymphangiogenesis and embryonic vascular development. It promotes endothelial cell function, angiogenesis, and forms a positive feedback loop that increases VEGFC production. VEGFR-3/FLT4 Protein, Mouse (HEK293, mFc) is the recombinant mouse-derived VEGFR-3/FLT4 protein, expressed by HEK293, with C-mFc labeled tag.
Background
VEGFR-3/FLT4 protein, a tyrosine-protein kinase, functions as a cell-surface receptor for VEGFC and VEGFD, playing a pivotal role in adult lymphangiogenesis and contributing significantly to the development of the vascular network and cardiovascular system during embryonic development. Its diverse functions include promoting the proliferation, survival, and migration of endothelial cells, along with the regulation of angiogenic sprouting. Activation of FLT4 leads to an augmented production of VEGFC and, to a lesser extent, VEGFA, establishing a positive feedback loop that enhances FLT4 signaling. Additionally, VEGFR-3/FLT4 modulates KDR signaling through the formation of heterodimers. Its signaling cascade involves the activation of the MAPK1/ERK2, MAPK3/ERK1, MAPK8, and JUN pathways, as well as the AKT1 pathway. VEGFR-3/FLT4 phosphorylates SHC1 and mediates the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Furthermore, it promotes the phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185,' as well as AKT1 at 'Ser-473,' underscoring its integral role in orchestrating complex signaling events crucial for lymphangiogenesis and vascular development.
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Immobilized Human VEGF-C Protein, His Tag at 5 μg/mL (100 μL/well) can bind Mouse VEGF R3 Protein, Mouse IgG2a Fc Tag with a linear range of 0.31-5 ng/mL.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-mFc
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Accession
P35917 (Y25-E775)
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Gene ID14257
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Molecular Construction
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N-term
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VEGFR-3 (Y25-E775)
Accession # P35917 -
mFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
FLT4; Fms Related Tyrosine Kinase 4; Fms Related Receptor Tyrosine Kinase 4; VEGF Receptor-3; VEGFR3; FLT-4; Vascular Endothelial Growth Factor Receptor 3; Vascular Endothelial Growth Factor Receptor 3 Variant; VEGFR-3; Alternative Protein FLT4; Tyrosine-
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AA Sequence
YSMTPPTLNITEDSYVIDTGDSLSISCRGQHPLEWTWPGAQEVLTTGGKDSEDTRVVHDCEGTEARPYCKVLLLAQTHANNTGSYHCYYKYIKARIEGTTAASTYVFVRDFKHPFINKPDTLLVNRKDSMWVPCLVSIPGLNITLRSQSSALHPDGQEVLWDDRRGMRVPTQLLRDALYLQCETTWGDQNFLSNLFVVHITGNELYDIQLYPKKSMELLVGEKLVLNCTVWAEFDSGVTFDWDYPGKQAERAKWVPERRSQQTHTELSSILTIHNVSQNDLGPYVCEANNGIQRFRESTEVIVHEKPFISVEWLKGPVLEATAGDELVKLPVKLAAYPPPEFQWYKDRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSAAGLRQNISLELVVNVPPHIHEKEASSPSIYSRHSRQTLTCTAYGVPQPLSVQWHWRPWTPCKTFAQRSLRRRQQRDGMPQCRDWKEVTTQDAVNPIESLDSWTEFVEGKNKTVSKLVIQDANVSAMYKCVVVNKVGQDERLIYFYVTTIPDGFSIESEPSEDPLEGQSVRLSCRADNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHLFATPLEANLEEAEPGARHATLSLNIPRVAPEDEGDYVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLTDLLVNVSDSLEMRCPVAGAHVPSIVWYKDERLLEKESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSEDKGSME
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Predicted Molecular Mass
111.5 kDa
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Molecular Weight
Approximately 116-125 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Monomer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 100 mM Glycine, pH 7.5 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (241 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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