Wnt4 protein, Human (HEK293, C-hFc)

The Wnt4 protein is a Frizzled receptor ligand that plays a critical role in embryonic urogenital tract and lung development. It is critical for embryonic kidney development and the formation of early renal vesicles and the mesenchymal to epithelial transition. Wnt4 protein, Human (HEK293, C-hFc) is the recombinant human-derived Wnt4 protein, expressed by HEK293 , with C-hFc labeled tag.

The Wnt4 protein operates as a ligand for members of the frizzled family of seven transmembrane receptors, playing a crucial role in the embryonic development of the urogenital tract and the lung. It is required for the normal mesenchyme to epithelium transition during embryonic kidney development and for the formation of early epithelial renal vesicles. Wnt4 is indispensable for the formation of the Mullerian duct in females and the maintenance of normal oocyte levels in the ovaries. Additionally, it is necessary for the normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Furthermore, Wnt4 is essential for normal lung development and the proper patterning of tracheal cartilage rings. The protein interacts with PORCN and PKD1, underscoring its intricate involvement in various developmental processes and signaling pathways.

Human

HEK293

C-hFc

P56705-1 (S23-R351)

54361  [NCBI]

Full Length of Isoform-1 Mature Protein

SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDMRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCSCKFHWCCFVKCRQCQRLVELHTCR

Approximately 60-68 kDa, based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.