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Wnt4 protein, Human (HEK293, C-hFc)

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The Wnt4 protein is a Frizzled receptor ligand that plays a critical role in embryonic urogenital tract and lung development. It is critical for embryonic kidney development and the formation of early renal vesicles and the mesenchymal to epithelial transition. Wnt4 protein, Human (HEK293, C-hFc) is the recombinant human-derived Wnt4 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The Wnt4 protein is a Frizzled receptor ligand that plays a critical role in embryonic urogenital tract and lung development. It is critical for embryonic kidney development and the formation of early renal vesicles and the mesenchymal to epithelial transition. Wnt4 protein, Human (HEK293, C-hFc) is the recombinant human-derived Wnt4 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

The Wnt4 protein operates as a ligand for members of the frizzled family of seven transmembrane receptors, playing a crucial role in the embryonic development of the urogenital tract and the lung. It is required for the normal mesenchyme to epithelium transition during embryonic kidney development and for the formation of early epithelial renal vesicles. Wnt4 is indispensable for the formation of the Mullerian duct in females and the maintenance of normal oocyte levels in the ovaries. Additionally, it is necessary for the normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Furthermore, Wnt4 is essential for normal lung development and the proper patterning of tracheal cartilage rings. The protein interacts with PORCN and PKD1, underscoring its intricate involvement in various developmental processes and signaling pathways.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    P56705-1 (S23-R351)

  • Gene ID
    54361  [NCBI]
  • Molecular Construction
    • N-term
    • Wnt4 (S23-R351)
      Accession # P56705-1
    • hFc
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    WNT4; Wnt Family Member 4; WNT-4; Wingless-Type MMTV Integration Site Family, Member 4; Protein Wnt-4; Protein Wnt; SERKAL

  • AA Sequence

    SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDMRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCSCKFHWCCFVKCRQCQRLVELHTCR

  • Predicted Molecular Mass

    65.6 kDa

  • Molecular Weight

    Approximately 60-68 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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