Rhizopodin
Rhizopodin is a new cytostatic compound isolated from the culture fluid of the slime mold Myxococcus stipitatus. Rhizopodin inhibits the growth of various animal cell cultures without killing the cells, with an ID50 value of 12 to 30 ng/ml, depending on the cell line. In particular, fibroblast-like cells show typical morphological changes, with cells becoming larger and forming long branched reticular extensions within hours, and these morphological changes are irreversible. Rhizopodin inhibits bleb formation in K-562 cells, possibly through interaction with protein phosphorylation.
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- CAS No.: 150346-23-9
- Formula: C78H124N4O22
- Molecular Weight:1469.83
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 150346-23-9
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Molecular Weight 1469.83
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Formula C78H124N4O22
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SMILES
CO[C@]1([H])C2=COC(C[C@@H](C(C)([C@@H](OC(C[C@H](C[C@H](/C=C/C=C/C[C@@](OC)([H])C3=COC(C[C@H](O)C(C)(C)[C@H](C[C@H](OC)[C@@H](C)CCC([C@H](C)[C@H](OC)C/C=C/N(C)C=O)=O)OC(C[C@@H](O)C[C@@H](OC)/C=C/C=C/C1)=O)=N3)OC)O)=O)C[C@H](OC)[C@@H](C)CCC([C@H](C)[C@H](OC)C/C=C/N(C)C=O)=O)C)O)=N2
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Structure Classification
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Initial Source
Myxococcus stipitatus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Kinase activity and phosphorylation assays
Kinase activity assays measure the ability of kinases to transfer phosphate groups from ATP to specific substrates, while phosphorylation assays detect the presence and levels of phosphorylated proteins. Common methods include radiolabeled ATP incorporation (e. g. ,), ADP release detection via bioluminescence (e. g. ,[3]), enzyme-linked immunosorbent assays (ELISA) for phospho-specific epitopes (e. g. ,[6]), and microtiter-based formats for high-throughput screening (e. g. ,[8]). The ADP-Glo assay quantifies kinase activity by measuring ADP produced during phosphorylation using a luciferase-based system. Radiometric assays involve autoradiography or scintillation counting after incorporation of 32P-labeled ATP into substrate proteins. ELISA-based approaches rely on phospho-specific antibodies to detect activated kinases in cell lysates or purified samples.
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
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Protocol for Kinase activity and phosphorylation assays
Kinase activity assays measure transfer of phosphate from ATP to a protein or peptide substrate, generating phosphorylated substrate, ADP, or incorporated radiolabeled phosphate as the readout; phosphorylation assays measure site-specific phosphorylation in cells or tissues as a proxy for kinase-pathway activation, inhibition, or substrate regulation. Phosphorylation can be detected by phospho-specific Western blot, immunoprecipitation kinase assay, phospho-immunofluorescence, phospho-flow cytometry, luminescent ADP detection, radiolabeled ATP incorporation, or reporter-based pathway assays, and these readouts can be applied to cancer cells, primary neurons, mouse tumors, organoids, inflammatory macrophages, ferroptosis studies, and mitophagy studies when the kinase target is biologically relevant.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Rhizopodin Related Classifications
HY-120883 Related Classifications
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