RNase H2
Based on 1 Customer Validation
RNase H2 is the predominant source of RNase H activity in mammalian and human cells. RNase H2 protects genome integrity. RNase H2 has been associated with ribonucleotide removal from genomic DNA in yeast and mouse, where it is required for embryonic development.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
Product Information
This product is recombinantly expressed in E. coli. In addition to degrading the RNA chain in the R-loop and DNA-RNA complex chain, it can also specifically cut single ribonucleotides in the DNA double-stranded chain to repair the DNA double-stranded chain.
Storage buffer: 20 mM Tris-HCl, 100 mM KCI, 1 mM DTT, 1 mM EDTA, 50% Glycerol, pH 8.0
Instructions
1. Add 100 pmol double-stranded DNA substrate, 0.2-0.5 μL RNase H2, 5 μL reaction buffer (200 mM Tris-HCl, 100 mM (NH4) 2SO4, 100 mM KCl, 20 mM MgSO4, 1% Triton X-100, pH 8.8) according to the reaction system, and add Nuclease-free Water to make up to 50 μL;
2. After mixing, incubate the sample at 37°C for 30 min.
| Specification | Type | Components | Volume | Quantity |
| 100 U | A | RNase H2 | 100 U | 1 |
| 100 U | B | 10x RNase H2 Reaction Buffer | 200 μL | 2 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.1.26.4
Enzyme Activity
>5 U/μl
Unit Definition
One unit is defined as the amount of enzyme that can cleave 1 nmol of synthetic DNA/RNA hybrid double-stranded body substrate containing a single R-loop per min at 37°C
化学情報
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性状 Liquid
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Color Colorless to light yellow
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SMILES
[RNase H2]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
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データシート (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Feng S, Cao Z. Is the role of human RNase H2 restricted to its enzyme activity? Prog Biophys Mol Biol. 2016 May;121(1):66-73. [Content Brief]
[2]. Chon H, et al. RNase H2 roles in genome integrity revealed by unlinking its activities. Nucleic Acids Res. 2013 Mar 1;41(5):3130-43. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
RNase H2 関連分類
HY-E70098 関連分類
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