Ruthenium Red Staining Solution (0.01%)
Ruthenium Red Staining Solution (0.01%) is a polycationic dye widely used for electron microscopy (EM) of cells, tissues and vegetative bacteria. Ruthenium Red Staining Solution (0.01%) strongly reacts with phospholipids and fatty acids and binds to acidic mucopolysaccharides. Ruthenium Red Staining Solution (0.01%) is a L-type calcium current (ICa) blocker. When using Ruthenium Red Staining Solution (0.01%) to stain plant tissue sections affected by pathogens, the breakdown of mucilaginous substances by the pathogens prevents them from taking up the stain, whereas the cell walls of healthy tissue stain red.
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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Activité biologique
ICa[2]
Ruthenium Red Staining Solution (0.01%) staining of intracellular organelles and structures seemed to depend on the time of exposure to this agent, as well on the concentration used[2].
Ruthenium Red Staining Solution (0.01%) efficiently blocks the L-type calcium current in a dose-dependent manner in in guinea-pig isolated ventricular heart cells, with EC50 of 0.8 μM[2].
Ruthenium Red Staining Solution (0.01%) (10 μM) blocks the sarcoplasmic Ca2+ release channels or the mitochondrial Ca2+ uptake, and blocks 26.7 % of the sodium current, and slows its inactivation time-course in guinea-pig isolated ventricular heart cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Lashanda N Waller, et al. Ruthenium red staining for ultrastructural visualization of a glycoprotein layer surrounding the spore of Bacillus anthracis and Bacillus subtilis. J Microbiol Methods. 2004 Jul;58(1):23-30. [Content Brief]
[2]. Claire O Malécot, et al. Ruthenium red as an effective blocker of calcium and sodium currents in guinea-pig isolated ventricular heart cells. Br J Pharmacol. 1998 Jun; 124(3): 465-472. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ruthenium Red Staining Solution (0.01%) Related Classifications
HY-DY2034 Related Classifications
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