Salivaricin B
Based on 1 Customer Validation
Salivaricin B is a broad-spectrum bacteriocin produced by Lactobacillus M7. Salivaricin B reduces cell wall thickness, induces abnormal septum formation, and exerts rapid bactericidal activity against susceptible Gram-positive bacteria without disrupting cytoplasmic membrane integrity or dissipating membrane potential. Salivaricin B inhibits the growth of oral, upper respiratory tract and food-related Gram-positive pathogens, spoilage bacteria, lactobacilli, and SboB-non-producing Streptococcus salivarius strains. Its activity is strain-dependent, and it has no effect on Gram-negative bacteria. Salivaricin B can be used in studies related to bacterial infections.
For research use only. We do not sell to patients.
- Purity : 92.35%
- CAS No.: 335080-41-6
- Formula: C120H182N34O36S4
- Molecular Weight:2805.20
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Salivaricin B (18 h) inhibits the growth of multiple susceptible Gram-positive bacterial strains with strain-dependent potency, ranging from an MIC of 269 nM (Corynebacterium spp GH17) to 4320 nM (Streptococcus sanguinis ATCC10556)[1].
Salivaricin B (10× MIC; up to 150 min) exhibits rapid bactericidal activity against Streptococcus pyogenes ATCC12344 and Micrococcus luteus ATCC10240, killing over 40% of cells within 30 minutes and over 90% within 3 hours without inducing significant lysis[1].
Salivaricin B (0.5-2.5 μM; 5 h for Streptococcus pyogenes, 0.125-0.5 μM; 10 h for Micrococcus luteus) exerts concentration-dependent growth inhibition against Streptococcus pyogenes ATCC12344 and Micrococcus luteus ATCC10240, with complete inhibition at 2.5 μM and 0.5 μM, respectively[1].
Salivaricin B (0.05 mM) interacts with Bacillus cereus and Escherichia coli membrane vesicles but does not penetrate the membranes, as indicated by the absence of a tryptophan fluorescence blue shift[1].
Salivaricin B (10× MIC; 60 min) induces accumulation of the cell wall precursor UDP-MurNAc-pentapeptide in Micrococcus luteus ATCC10240, indicating interference with peptidoglycan biosynthesis[1].
Salivaricin B (10× MIC; 30 min, 120 min, or overnight) induces ultrastructural changes including reduced cell wall thickness and aberrant septum formation in Streptococcus pyogenes ATCC12344 and Micrococcus luteus ATCC10240, without causing membrane damage[1].
Salivaricin B exhibits broad-spectrum antimicrobial activity against Gram-positive pathogens, spoilage organisms, and multiple lactobacilli[2].
Salivaricin B (20% (v/v) culture supernatant; incubation at 37°C until viability was measured) (from Lactobacillus salivarius M7) exerts a bactericidal effect on Lactobacillus bulgaricus ATCC 11842, reducing viable cell counts by 100-fold without causing cell lysis[2].
Salivaricin B (18-24 h) inhibits the growth of Micrococcus varians 1482, Lactococcus lactis C2102, and 14 of 25 tested Streptococcus salivarius strains, while showing no self-inhibition[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 335080-41-6
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Appearance Solid
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Molecular Weight 2805.20
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Formula C120H182N34O36S4
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Color White to off-white
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Sequence
Gly-Gly-Gly-Val-Ile-Gln-Thr-Ile-Ser-His-Glu-Cys-Arg-Met-Asn-Ser-Trp-Gln-Phe-Leu-Phe-Thr-Cys-Cys-Ser
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Sequence Shortening
GGGVIQTISHECRMNSWQFLFTCCS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 20 mg/mL (7.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2 mg/mL (0.71 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Fluorescent plasma-membrane potential dye assay
Fluorescent plasma-membrane potential dye assays measure changes in cell membrane potential using voltage-sensitive dyes whose fluorescence changes when cells depolarize or hyperpolarize. Anionic bis-oxonol dyes such as DiBAC4(3) enter depolarized cells more readily and show increased fluorescence after intracellular binding, while hyperpolarization reduces dye accumulation and fluorescence. FMP/FLIPR membrane-potential dyes are used for faster, homogeneous microplate assays of ion-channel or receptor-mediated membrane-potential changes.
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
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Bacterial live/dead nucleic-acid viability staining
The LIVE/DEAD bacterial viability staining method is based on differential permeability of nucleic-acid-binding fluorescent dyes, most commonly SYTO 9 and propidium iodide (PI), which enables discrimination of bacterial populations with intact versus compromised cytoplasmic membranes. SYTO 9 penetrates both intact and damaged bacterial membranes and binds nucleic acids to produce green fluorescence, whereas propidium iodide penetrates only cells with compromised membranes and fluoresces red while also reducing SYTO 9 signal through competitive binding and fluorescence interactions. The resulting fluorescence pattern is interpreted as a proxy for membrane integrity, which is widely used as an indicator of bacterial viability in microscopy, flow cytometry, and spectroscopic platforms. However, mechanistic studies show that SYTO 9 and PI interactions involve displacement and fluorescence resonance energy transfer effects, which can influence signal interpretation depending on dye ratios a
Purity & Documentation
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Data Sheet (290 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.3565 mL | 1.7824 mL | 3.5648 mL | 8.9120 mL |
| 5 mM | 0.0713 mL | 0.3565 mL | 0.7130 mL | 1.7824 mL |
Salivaricin B Related Classifications
HY-P10134 Related Classifications
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