Schisanlactone D
Schisanlactone D is an FXR inhibitor. Schisanlactone D inhibits the proliferation of B16 mouse melanoma cells. Schisanlactone D can be used for research on melanoma.
For research use only. We do not sell to patients.
- CAS No.: 92051-26-8
- Formula: C30H44O3
- Molecular Weight:452.68
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| B16 | IC50 |
8.56 μM
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Antiproliferative activity against mouse B16 melanoma cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay.
Antiproliferative activity against mouse B16 melanoma cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay.
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27425445 |
In Vitro
Schisanlactone D (0-50 μM; 24 h) exhibits potent cytotoxicity against B16 mouse melanoma cells with an IC50 of 8.56 μM[1].
Schisanlactone D is a triterpenoid with the molecular formula C32H50O5, structurally characterized as an ethyl ester derivative of Schinalactone C[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:B16
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Concentration:0, 1, 4, 8, 20, and 50 μM
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Incubation Time:24 h
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Result:Exhibited an IC50 value of 8.56 μM.
Chemical Information
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CAS No. 92051-26-8
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Molecular Weight 452.68
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Formula C30H44O3
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SMILES
C[C@]12[C@]3(C([C@]4(C)[C@@](CC3)(C(C)(C)C(=O)CC4)[H])=CC[C@]1(C)[C@@]([C@H](C)[C@@]5(OC(=O)C(C)=CC5)[H])(CC2)[H])[H]
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Schisanlactone D Related Classifications
HY-N21663 Related Classifications
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