Cypyrafluone
Based on 1 Customer Validation
Cypyrafluone (Huanbifucaotong) is a HPPD inhibitor and herbicide with an IC50 of 0.70 µM against AtHPPD. Cypyrafluone blocks the biosynthesis of plastoquinone and α-tocopherol through targeted inhibition of HPPD enzyme activity, thereby leading to the loss of photosynthetic pigments and triggering ROS-mediated photooxidative damage. Cypyrafluone can be used in research on agricultural herbicides.
For research use only. We do not sell to patients.
- Purity : 99.58%
- CAS No.: 1855929-45-1
- Formula: C20H19ClF3N3O3
- Molecular Weight:441.83
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
AtHPPD 0.7 μM (IC50) |
ZtHPPD 959 nM (IC50) |
In Vitro
Cypyrafluone (different concentrations; 10-20 min at 30 °C) is an in vitro inhibitor of Arabidopsis thaliana HPPD with an IC50 of 0.70 μM[2].
Cypyrafluone (0-160 g a.i. ha-1; 21 DAT) controls Alopecurus japonicus with a GR50 of 22.44 g a.i. ha-1[2].
Cypyrafluone (90 g a.i. ha-1; 5-7 DAT) causes phytoene accumulation and reduces carotenoid and chlorophyll pigments in Alopecurus japonicus leaves[2].
Cypyrafluone (25 mg L-1; 7 DAT) decreases Spirodela polyrhiza chlorophyll content, and HGA addition reverses this decrease[2].
Cypyrafluone (150 g a.i. ha-1; 3-21 DAT) causes bleaching and death in wild-type rice, but OsHPPD-overexpressing rice tolerates it[2].
Cypyrafluone (0-4860 g a.i. ha-1; 21 DAT) kills wild-type Arabidopsis thaliana at 30 g a.i. ha-1, while OsHPPD-overexpressing A. thaliana tolerates up to 4860 g a.i. ha-1, with over 90% fresh weight inhibition at the highest rate[2].
Cypyrafluone inhibits purified ZtHPPD with an IC50 of 959 nM in the coupled enzyme HPPD inhibition assay[3].
Cypyrafluone (21 d) remains highly effective against both R-06 and S-19 Alopecurus myosuroides populations, with IR > 96%[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Alopecurus japonicus
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Concentration:5, 10, 20, 40, 80, and 160 g
a.i. ha−1 -
Incubation Time:21 days
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Result:Controled Alopecurus japonicus, with a GR50 of 22.44 g a.i. ha⁻¹.
Chemical Information
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CAS No. 1855929-45-1
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Appearance Solid
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Molecular Weight 441.83
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Formula C20H19ClF3N3O3
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Color White to off-white
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SMILES
O=C(C1=C(N(C)N=C1C2CC2)O)C3=CC=C(C(F)(F)F)C(N4C(CCCC4)=O)=C3Cl
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Synonyms
Huanbifucaotong
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (113.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.83 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.83 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (294 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2633 mL | 11.3166 mL | 22.6331 mL | 56.5829 mL |
| 5 mM | 0.4527 mL | 2.2633 mL | 4.5266 mL | 11.3166 mL | |
| 10 mM | 0.2263 mL | 1.1317 mL | 2.2633 mL | 5.6583 mL | |
| 15 mM | 0.1509 mL | 0.7544 mL | 1.5089 mL | 3.7722 mL | |
| 20 mM | 0.1132 mL | 0.5658 mL | 1.1317 mL | 2.8291 mL | |
| 25 mM | 0.0905 mL | 0.4527 mL | 0.9053 mL | 2.2633 mL | |
| 30 mM | 0.0754 mL | 0.3772 mL | 0.7544 mL | 1.8861 mL | |
| 40 mM | 0.0566 mL | 0.2829 mL | 0.5658 mL | 1.4146 mL | |
| 50 mM | 0.0453 mL | 0.2263 mL | 0.4527 mL | 1.1317 mL | |
| 60 mM | 0.0377 mL | 0.1886 mL | 0.3772 mL | 0.9430 mL | |
| 80 mM | 0.0283 mL | 0.1415 mL | 0.2829 mL | 0.7073 mL | |
| 100 mM | 0.0226 mL | 0.1132 mL | 0.2263 mL | 0.5658 mL |
Cypyrafluone Related Classifications
HY-W725385 Related Classifications
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