SGC5 

SGC5 is a fluorescence enhancer and membrane dissociator with millisecond-scale membrane dissociation kinetics, with an emission spectrum of 500-650 nm. SGC5 produces strong fluorescence upon binding to lipid membranes, exhibits fast partitioning dissociation kinetics at higher temperatures, and is unaffected by pH or cholesterol. SGC5 does not belong to the FM dye family and shows the highest membrane-bound fluorescence intensity among the tested styryl/FM dyes. SGC5 can be used to study synaptic vesicle cycling in neurons^[1].

When SGC5 (0-20 μM) binds to 30% PE/70% PC liposomes, its fluorescence intensity is higher than that of all tested FM dyes, and the fold increase in fluorescence after membrane binding is second only to that of FM1-84^[1].
SGC5 (0-20 nM liposomes; fixed SGC5 concentration) binds to 30% PE/70% PC liposomes with an EC₅₀ of 4.0 nM, and the resulting fluorescence intensity and signal-to-noise ratio are comparable to those of FM1-84 (HY-D1515) and FM1-43 (HY-D1434)^[1].
After being loaded into synaptic vesicles of cultured rat hippocampal neurons, SGC5 (2 μM; 3 min) emits a peak at ~530 nm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

681.58

C₃₃H₅₁Br₂N₃O₂

1331918-67-2

CC[N+](CCC[N+]1=CC=C(C(C2=O)=CC3=C(O2)C=C(N(CCCCC)CCCCC)C=C3)C=C1)(CC)CC.[Br-].[Br-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wu Y, et al. Biophysical characterization of styryl dye-membrane interactions[J]. Biophysical journal, 2009, 97(1): 101-109.