Simmon's Citrate Agar
Simmon's Citrate Agar is a synthetic differential medium that uses sodium citrate as the sole carbon source. Simmon's Citrate Agar can determine the ability of bacteria to utilize citrate as a carbon source. Bacterial metabolism of citrate produces bicarbonate, which causes bromothymol blue to change from blue to deep blue. Simmon's Citrate Agar can be used for bacterial identification and isolation-related research.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Simmon's Citrate Agar can serve as the basal medium for preparing SCITB agar, which is used to identify and isolate K. oxytoca from diarrheal stool specimens[1].
Simmon's Citrate Agar supports colicin production in 28 of 73 colicinogenic strains, among which colicine I, colicine G, and S5 strains consistently fail to produce colicin in this synthetic medium, whereas colicines V, K, X, D, and F produce large inhibition zones[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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SMILES
[Simmon's Citrate Agar]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Selective and Differential Bacterial Isolation Culture
Selective and differential bacterial isolation culture separates viable bacteria by plating specimens on solid media that both suppress unwanted organisms and display interpretable colony phenotypes. MacConkey-type media use bile salts to support intestinal Gram-negative organisms while distinguishing lactose-fermenting bacteria in fecal material; sorbitol-MacConkey replaces lactose with sorbitol to detect non-sorbitol-fermenting Escherichia coli O157:H7; mannitol salt agar selectively recovers Staphylococcus aureus and differentiates yellow mannitol-positive colonies; chromogenic orientation media use enzyme-substrate color reactions to presumptively distinguish uropathogens on primary plates.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Simmon's Citrate Agar Related Classifications
HY-189512 Related Classifications
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