Sulfo-Cy5-N3
Based on 1 Customer Validation
Sulfo-Cy5-N3 is an azide-functionalized sulfonated cyanine 5 Fluorescent dye and also serves as click chemistry coupling reagent. Sulfo-Cy5-N3 undergoes copper (I)-catalyzed azide-alkyne cycloaddition with alkyne-functionalized antibody constructs and peptide probes for site-selective fluorescent labeling. Sulfo-Cy5-N3 also acts as an emission dye for STORM super-resolution imaging. Sulfo-Cy5-N3 is applicable to the study of HER2-positive breast cancer.
For research use only. We do not sell to patients.
- Purity : 98.88%
- CAS No.: 1481447-40-8
- Formula: C35H44N6O7S2
- Molecular Weight:724.89
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Sulfo-Cy5-N3 (16 h) efficiently conjugates via CuAAC to all four terminal alkyne handles of Astra-PD-bridged full-length Herceptin, yielding a homogeneous, stable dual-modified antibody-agent-fluorophore conjugate with preserved HER2 binding specificity and intact antibody structure[1].
Sulfo-Cy5-N3 is an azide-functionalized sulfonated cyanine5 fluorophore with an exact mass of 725.28 Da, classified as a dSTORM-compatible photoswitchable dye[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1481447-40-8
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Appearance Solid
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Molecular Weight 724.89
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Formula C35H44N6O7S2
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Color Brown to dark brown
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SMILES
O=S(C1=CC2=C([N+](C)=C(C=CC=CC=C3N(CCCCCC(NCCCN=[N+]=[N-])=O)C4=C(C=C(S(=O)(O)=O)C=C4)C3(C)C)C2(C)C)C=C1)([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (172.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3795 mL | 6.8976 mL | 13.7952 mL | 34.4880 mL |
| 5 mM | 0.2759 mL | 1.3795 mL | 2.7590 mL | 6.8976 mL | |
| 10 mM | 0.1380 mL | 0.6898 mL | 1.3795 mL | 3.4488 mL | |
| 15 mM | 0.0920 mL | 0.4598 mL | 0.9197 mL | 2.2992 mL | |
| 20 mM | 0.0690 mL | 0.3449 mL | 0.6898 mL | 1.7244 mL | |
| 25 mM | 0.0552 mL | 0.2759 mL | 0.5518 mL | 1.3795 mL | |
| 30 mM | 0.0460 mL | 0.2299 mL | 0.4598 mL | 1.1496 mL | |
| 40 mM | 0.0345 mL | 0.1724 mL | 0.3449 mL | 0.8622 mL | |
| 50 mM | 0.0276 mL | 0.1380 mL | 0.2759 mL | 0.6898 mL | |
| 60 mM | 0.0230 mL | 0.1150 mL | 0.2299 mL | 0.5748 mL | |
| 80 mM | 0.0172 mL | 0.0862 mL | 0.1724 mL | 0.4311 mL | |
| 100 mM | 0.0138 mL | 0.0690 mL | 0.1380 mL | 0.3449 mL |
Sulfo-Cy5-N3 Related Classifications
HY-W400925 Related Classifications
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Keywords
- Sulfo-Cy5-N3
- 1481447-40-8
- Fluorescent Dye
- azide-functionalized sulfonated cyanine5 fluorophore
- STORM super-resolution imaging
- peptide probes
- click chemistry conjugation reagent
- her2-positive breast cancer
- site-selective fluorescent labelling
- Herceptin Fab fragment
- alkyne-functionalized antibody constructs
- dSTORM-compatible photoswitchable dye
- copper(I)-catalysed azide-alkyne cycloaddition
- Inhibitor
- inhibitor
- inhibit