Sulfo-NHS-Biotin sodium

Name: Sulfo-NHS-Biotin sodium
Brand: MedChemExpress
SKU: HY-136964
Rating: 4.5 (1 reviews)

Cat. No.: HY-136964 Purity: 94.50%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Sulfo-NHS-Biotin (sodium) stongly label single SDS-2ME soluble cuticular protein.

For research use only. We do not sell to patients.

Sulfo-NHS-Biotin sodium Chemical Structure

CAS No. : 194041-65-1

Size	Price	Stock	Quantity
10 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 115	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

Sulfo-NHS-Biotin (sodium) stongly label single SDS-2ME soluble cuticular protein^[1].

Molecular Weight

443.43

Formula

C₁₄H₁₈N₃NaO₈S₂

CAS No.

194041-65-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(ON(C(C1)=O)C(C1[S](=O)(O[Na])=O)=O)CCCC[C@H]2[C@](N3)([H])[C@@](CS2)([H])NC3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (563.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2551 mL	11.2757 mL	22.5515 mL
5 mM	0.4510 mL	2.2551 mL	4.5103 mL
10 mM	0.2255 mL	1.1276 mL	2.2551 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 94.50%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (249 KB) HNMR (294 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Hill DE, et al. Biotin as a probe of the surface of Ascaris suum developmental stages. Mol Biochem Parasitol. 1990;41(1):45-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2551 mL	11.2757 mL	22.5515 mL	56.3787 mL
	5 mM	0.4510 mL	2.2551 mL	4.5103 mL	11.2757 mL
	10 mM	0.2255 mL	1.1276 mL	2.2551 mL	5.6379 mL
	15 mM	0.1503 mL	0.7517 mL	1.5034 mL	3.7586 mL
	20 mM	0.1128 mL	0.5638 mL	1.1276 mL	2.8189 mL
	25 mM	0.0902 mL	0.4510 mL	0.9021 mL	2.2551 mL
	30 mM	0.0752 mL	0.3759 mL	0.7517 mL	1.8793 mL
	40 mM	0.0564 mL	0.2819 mL	0.5638 mL	1.4095 mL
	50 mM	0.0451 mL	0.2255 mL	0.4510 mL	1.1276 mL
	60 mM	0.0376 mL	0.1879 mL	0.3759 mL	0.9396 mL
	80 mM	0.0282 mL	0.1409 mL	0.2819 mL	0.7047 mL
	100 mM	0.0226 mL	0.1128 mL	0.2255 mL	0.5638 mL