2. Autophagy
  3. Autophagy
  4. T1742

T1742 is an autophagy inhibitor targeting ATG5. T1742 blocks and disrupts protein-protein interactions between ATG5 and ATG16L1, and between ATG5 and TECAIR.T 1742 inhibits autophagy in living cells via reduced autophagosome formation and downregulated ATG8-PE conjugation. T1742 can be used to study autophagy mechanisms.

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T1742

T1742 Chemical Structure

CAS No. : 2841473-81-0

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T1742 Related Antibodies

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Description

T1742 is an autophagy inhibitor targeting ATG5. T1742 blocks and disrupts protein-protein interactions between ATG5 and ATG16L1, and between ATG5 and TECAIR.T 1742 inhibits autophagy in living cells via reduced autophagosome formation and downregulated ATG8-PE conjugation. T1742 can be used to study autophagy mechanisms[1][2][3].

In Vitro

T1742 (2.5-40 μM; 16 h) dose-dependently inhibits autophagy in COS-7 cells[1].
T1742 (1.25-40 μM; 6 h) dose-dependently reduces LC3-II levels, indicating autophagy inhibition, in COS-7, U-937, and THP-1 cells[1].
T1742 blocks the ATG5-ATG16L1 and ATG5-TECAIR protein-protein interactions in vitro with IC50 values of 1.1 μM and 1.7 μM, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

T1742 Related Antibodies

Cell Autophagy Assay[1]

Cell Line: COS-7
Concentration: 2.5; 5; 10; 20; 40 μM
Incubation Time: 16 h
Result: Caused a dose-dependent left shift in LC3 fluorescence intensity, indicating autophagy inhibition.

Western Blot Analysis[1]

Cell Line: COS-7, U-937, THP-1
Concentration: 1.25; 2.5; 5; 10; 20 μM (COS-7 cells); 5; 10; 20; 40 μM (U-937 and THP-1 cells)
Incubation Time: 6 h
Result: Reduced the LC3-II/β-actin ratio from 1.01 at 1.25 μM to 0.64 at 20 μM in COS-7 cells.
Caused a significant dose-dependent decrease in LC3-II protein levels in U-937 and THP-1 cells.
Molecular Weight

430.41

Formula

C24H18N2O6
CAS No.
SMILES

OC(CCN1C(C2=CC=CC=C2)=C/C(C1=O)=C\C3=CC=C(C4=CC=C(C=C4)[N+]([O-])=O)O3)=O
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Room temperature in continental US; may vary elsewhere.
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Purity & Documentation
References
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T1742 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

T17422841473-81-0T 1742T-1742AutophagyCOS-7 cellsautophagosomeTHP-1 cellsATG16L1autophagyATG8-PE conjugationATG5ATG5-TECAIR complexTECAIRU-937 cellsInhibitorinhibitorinhibit

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