Tesirine intermediate-2
Based on 1 Customer Validation
Tesirine intermediate-2 is the intermediate of Tesirine (HY-128952). Tesirine (SG3249), a pyrrole benzodiazepine (PBD) dimer, is a DNA small channel crosslinker with strong cytotoxicity. Tesirine can be used to synthesize Antibody-Drug Conjugates (ADCs), the warhead component of the payload is SG3199 (HY-101161), which has strong anticancer cell activity.
For research use only. We do not sell to patients.
- Purity : 99.43%
- CAS No.: 1430738-34-3
- Formula: C40H55N5O10Si
- Molecular Weight:793.98
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-101160
SG2057PBD Dimer -
HY-34758
N-Nitroso-N-methylureaCarcinogen Agent -
HY-111375
AzoxymethaneGenotoxic Carcinogen -
HY-128612
MethylnitronitrosoguanidineAlkylating Agent -
HY-13585
CarmustineDNA/RNA Alkylator -
HY-17420A
Cyclophosphamide hydrateAlkylating Agent -
HY-13593
ChlorambucilDNA Alkylator -
HY-101161
SG3199PBD Dimer -
HY-129241
AGX51ID Protein Antagonist -
HY-13424
RITAp53-HDM-2 Interaction Inhibitor -
HY-13747
SemustineAntitumor Alkylating Agent -
HY-13543
TretazicarDNA Cross-linking Agent
Biological Activity
Description
Chemical Information
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CAS No. 1430738-34-3
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Appearance Solid
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Molecular Weight 793.98
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Formula C40H55N5O10Si
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Color White to off-white
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SMILES
CC(C)(C)[Si](C)(C)O[C@@H]1N(C2=CC(O)=C(OC)C=C2C(N3[C@@]1([H])CC(C)=C3)=O)C(OCC4=CC=C(C=C4)NC([C@H](C)NC([C@H](C(C)C)NC(OCC=C)=O)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 200 mg/mL (251.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.2595 mL | 6.2974 mL | 12.5948 mL | 31.4869 mL |
| 5 mM | 0.2519 mL | 1.2595 mL | 2.5190 mL | 6.2974 mL | |
| 10 mM | 0.1259 mL | 0.6297 mL | 1.2595 mL | 3.1487 mL | |
| 15 mM | 0.0840 mL | 0.4198 mL | 0.8397 mL | 2.0991 mL | |
| 20 mM | 0.0630 mL | 0.3149 mL | 0.6297 mL | 1.5743 mL | |
| 25 mM | 0.0504 mL | 0.2519 mL | 0.5038 mL | 1.2595 mL | |
| 30 mM | 0.0420 mL | 0.2099 mL | 0.4198 mL | 1.0496 mL | |
| 40 mM | 0.0315 mL | 0.1574 mL | 0.3149 mL | 0.7872 mL | |
| 50 mM | 0.0252 mL | 0.1259 mL | 0.2519 mL | 0.6297 mL | |
| 60 mM | 0.0210 mL | 0.1050 mL | 0.2099 mL | 0.5248 mL | |
| 80 mM | 0.0157 mL | 0.0787 mL | 0.1574 mL | 0.3936 mL | |
| 100 mM | 0.0126 mL | 0.0630 mL | 0.1259 mL | 0.3149 mL |
Tesirine intermediate-2 Related Classifications
HY-24144 Related Classifications
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