Tiadenol
Based on 1 Customer Validation
Tiadenol is an orally effective lipid-lowering compound and peroxisome proliferator. Tiadenol promotes peroxisome-associated fatty acid metabolism, and its induction of the activities of enzymes such as palmitoyl-CoA hydrolase, carnitine acetyl-transferase and catalase is mainly associated with de novo protein synthesis. The triglyceride-lowering effect of Tiadenol is not accompanied by increased activities of lipoprotein lipase (LPL) and hepatic lipase (HL), and it reduces VLDL Apo E. Tiadenol can be used in studies related to lipid metabolism, peroxisome proliferation and hyperlipoproteinemia.
For research use only. We do not sell to patients.
- Purity : 99.90%
- CAS No.: 6964-20-1
- Formula: C14H30O2S2
- Molecular Weight:294.52
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Tiadenol (500 mg/kg; p.o.; daily; 7 days) increases liver weight and catalase activity, decreases plasma cholesterol and total lipids, reduces biliary cholesterol concentration and output, increases bile flow and the cholesterol solubility ratio, and elevates the bile salt-independent fraction of bile secretion in normolipidemic rats[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar (male, 250-270 g)[3]
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Dosage:0.27 g/day/kg body weight
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Administration:p.o.; daily; 10 days
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Result:Increased liver weight, hepatic protein, free CoASH, and long-chain acyl-CoA.
Increased palmitoyl-CoA hydrolase, peroxisomal palmitoyl-CoA oxidation, and total catalase activity.
Induced marked redistribution of several hepatic peroxisomal/lipid-metabolizing enzyme activities among subcellular fractions.
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Animal Model:Wistar (male)[1]
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Dosage:500 mg/kg
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Administration:p.o.; daily; 7 days
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Result:Decreased plasma cholesterol and total plasma lipids.
Increased liver weight and liver catalase.
Increased basal bile flow.
Decreased biliary bile salt, phospholipid, and cholesterol concentrations and cholesterol output.
Chemical Information
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CAS No. 6964-20-1
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Appearance Solid
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Molecular Weight 294.52
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Formula C14H30O2S2
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SMILES
OCCSCCCCCCCCCCSCCO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (339.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (16.98 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (16.98 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
[1]. Rozé C, et al. The effects of tiadenol, clofibrate and clofibride on bile composition in the rat. European journal of pharmacology. 1977 May 01;43(1):57-64. [Content Brief]
[2]. Franceschini G, et al. Pharmacological studies on tiadenol in type IV patients: Evidence for a mechanism of action different from other lipid-lowering drugs. Atherosclerosis. 1981;40:245-255. [Content Brief]
[4]. Berge RK, et al. Tiadenol-mediated induction of peroxisomal enzymes in cultured C3H/10T1/2 CL8 cells and in chemically transformed C3H/10T1/2 MCA16 cells. Int J Cancer. 1985;36:489-494. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.3954 mL | 16.9768 mL | 33.9536 mL | 84.8839 mL |
| 5 mM | 0.6791 mL | 3.3954 mL | 6.7907 mL | 16.9768 mL | |
| 10 mM | 0.3395 mL | 1.6977 mL | 3.3954 mL | 8.4884 mL | |
| 15 mM | 0.2264 mL | 1.1318 mL | 2.2636 mL | 5.6589 mL | |
| 20 mM | 0.1698 mL | 0.8488 mL | 1.6977 mL | 4.2442 mL | |
| 25 mM | 0.1358 mL | 0.6791 mL | 1.3581 mL | 3.3954 mL | |
| 30 mM | 0.1132 mL | 0.5659 mL | 1.1318 mL | 2.8295 mL | |
| 40 mM | 0.0849 mL | 0.4244 mL | 0.8488 mL | 2.1221 mL | |
| 50 mM | 0.0679 mL | 0.3395 mL | 0.6791 mL | 1.6977 mL | |
| 60 mM | 0.0566 mL | 0.2829 mL | 0.5659 mL | 1.4147 mL | |
| 80 mM | 0.0424 mL | 0.2122 mL | 0.4244 mL | 1.0610 mL | |
| 100 mM | 0.0340 mL | 0.1698 mL | 0.3395 mL | 0.8488 mL |
Tiadenol Related Classifications
HY-127055 Related Classifications
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