TLR7 agonist 20 hydrochloride

Name: TLR7 agonist 20 hydrochloride
Brand: MedChemExpress
SKU: HY-158694A
Rating: 4.5 (0 reviews)

Cat. No.: HY-158694A Purity: 99.86%: Data Sheet
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TLR7 agonist 20 hydrochloride is a selective Toll-like receptor 7 (TLR7) agonist with an EC₅₀ of 0.22 μM. TLR7 agonist 20 hydrochloride, as an immunostimulant and vaccine adjuvant, enhances the production of anti-spike protein antibodies, increases IgG2b and IgG2c levels, and thereby drives Th1-type immune responses in mice. TLR7 agonist 20 hydrochloride can be used in studies related to non-inflammatory potent vaccine adjuvants.

For research use only. We do not sell to patients.

TLR7 agonist 20 hydrochloride Chemical Structure

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5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
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100 mg	In-stock	Estimated Time of Arrival: December 31
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Other Forms of TLR7 agonist 20 hydrochloride:

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

TLR7

0.22 μM (EC₅₀)

In Vitro

TLR7 agonist 20 (Compound 23) (≤ 10 μM) hydrochloride shows no cytotoxicity in the NCI60 human cancer cell line panel^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

TLR7 agonist 20 (Compound 23) (10 μg; i.m.; 2 doses) hydrochloride acts as a potent vaccine adjuvant in mice, inducing a strong Th1 response when administered alone with SARS-CoV-2 spike antigen and enhancing balanced Th1/Th2 responses when co-formulated with alum^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 (female, 6-8 weeks old)^[1]
Dosage:	10 μg (co-formulated with SARS-CoV-2 spike antigen); 10 μg (co-formulated with SARS-CoV-2 spike antigen and 50 μg alum)
Administration:	i.m.; administered at week 0 and week 2
Result:	Induced significantly higher anti-spike IgG2b (OD₄₅₀ ≈ 0.9) and IgG2c (OD₄₅₀ ≈ 0.8) levels compared to spike antigen alone. Induced significantly higher anti-spike IgG (OD₄₅₀ ≈ 2.5), IgG1 (OD₄₅₀ ≈ 3.0), IgG2b (OD₄₅₀ ≈ 0.7), and IgG2c (OD₄₅₀ ≈ 0.8) levels compared to spike antigen alone when co-formulated with alum and spike antigen.

Molecular Weight

396.91

Formula

C₂₂H₂₅ClN₄O

Appearance

Solid

Color

White to off-white

SMILES

CCCCC1=NC2=C(C3=CC=CC=C3N=C2N)N1CC4=CC=C(C=C4)CO.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (25.19 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5195 mL	12.5973 mL	25.1946 mL
5 mM	0.5039 mL	2.5195 mL	5.0389 mL
10 mM	0.2519 mL	1.2597 mL	2.5195 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.86%

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Data Sheet (275 KB) SDS (251 KB)

COA (252 KB) HNMR (273 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Kaushik D, et al. Structure-Activity Relationships toward the Identification of a High-Potency Selective Human Toll-like Receptor-7 Agonist. J Med Chem. 2024;67(10):8346-8360. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5195 mL	12.5973 mL	25.1946 mL	62.9866 mL
	5 mM	0.5039 mL	2.5195 mL	5.0389 mL	12.5973 mL
	10 mM	0.2519 mL	1.2597 mL	2.5195 mL	6.2987 mL
	15 mM	0.1680 mL	0.8398 mL	1.6796 mL	4.1991 mL
	20 mM	0.1260 mL	0.6299 mL	1.2597 mL	3.1493 mL
	25 mM	0.1008 mL	0.5039 mL	1.0078 mL	2.5195 mL