TLR7 agonist 20 hydrochloride
Based on 1 Customer Validation
TLR7 agonist 20 hydrochloride is a selective Toll-like receptor 7 (TLR7) agonist with an EC50 of 0.22 μM. TLR7 agonist 20 hydrochloride, as an immunostimulant and vaccine adjuvant, enhances the production of anti-spike protein antibodies, increases IgG2b and IgG2c levels, and thereby drives Th1-type immune responses in mice. TLR7 agonist 20 hydrochloride can be used in studies related to non-inflammatory potent vaccine adjuvants.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.86%
- Fòrmula: C22H25ClN4O
- Peso molecular:396.91
-
Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
Descripciòn
IC50 & Target
[1]|
TLR7 0.22 μM (EC50) |
In Vitro
TLR7 agonist 20 (Compound 23) (≤ 10 μM) hydrochloride shows no cytotoxicity in the NCI60 human cancer cell line panel[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:C57BL/6 (female, 6-8 weeks old)[1]
-
Dosage:10 μg (co-formulated with SARS-CoV-2 spike antigen); 10 μg (co-formulated with SARS-CoV-2 spike antigen and 50 μg alum)
-
Administration:i.m.; administered at week 0 and week 2
-
Result:Induced significantly higher anti-spike IgG2b (OD450 ≈ 0.9) and IgG2c (OD450 ≈ 0.8) levels compared to spike antigen alone.
Induced significantly higher anti-spike IgG (OD450 ≈ 2.5), IgG1 (OD450 ≈ 3.0), IgG2b (OD450 ≈ 0.7), and IgG2c (OD450 ≈ 0.8) levels compared to spike antigen alone when co-formulated with alum and spike antigen.
Chemical Information
-
Appearance Solid
-
Peso molecular 396.91
-
Fòrmula C22H25ClN4O
-
Color White to off-white
-
SMILES
CCCCC1=NC2=C(C3=CC=CC=C3N=C2N)N1CC4=CC=C(C=C4)CO.[H]Cl
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
In Vitro:
DMSO : 10 mg/mL (25.19 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocolo
-
Naïve CD4+ T-cell subset differentiation/polarization
Naïve CD4+ T-cell subset differentiation/polarization is an in vitro assay in which purified naïve CD4+ T cells are activated through TCR and CD28 costimulation and cultured with defined cytokines and neutralizing antibodies to generate Th0, Th1, Th2, Th17, or induced Treg-like populations. Differentiation is detected by subset-associated cytokines and transcription factors: IFN-γ/T-bet for Th1, IL-4/GATA3 for Th2, IL-17A/RORγt for Th17, and Foxp3 for induced Treg cells. The assay readout is usually generated by intracellular cytokine staining after restimulation, transcription-factor staining by flow cytometry, ELISA of secreted cytokines, or gene-expression analysis. The result reflects cytokine-directed lineage commitment or polarization rather than antigen-specific immune protection by itself.
Pureza y Documentación
-
Ficha de datos (275 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5195 mL | 12.5973 mL | 25.1946 mL | 62.9866 mL |
| 5 mM | 0.5039 mL | 2.5195 mL | 5.0389 mL | 12.5973 mL | |
| 10 mM | 0.2519 mL | 1.2597 mL | 2.5195 mL | 6.2987 mL | |
| 15 mM | 0.1680 mL | 0.8398 mL | 1.6796 mL | 4.1991 mL | |
| 20 mM | 0.1260 mL | 0.6299 mL | 1.2597 mL | 3.1493 mL | |
| 25 mM | 0.1008 mL | 0.5039 mL | 1.0078 mL | 2.5195 mL |
TLR7 agonist 20 hydrochloride Related Classifications
HY-158694A Related Classifications
Powered by Bioz