Trametenolic acid
Based on 1 Customer Validation
Trametenolic acid is a lanostanol glycoside that isolated from the EtOH extract of the fruit bodies of Laetiporus versisporus.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.28%
- CAS 番号: 24160-36-9
- 分子式: C30H48O3
- 分子量:456.70
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
[1]|
ERK1 |
ERK2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Ca-Ski | ED50 |
6.2 μg/mL
Compound: 8
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Cytotoxicity against human CaSKi cells after 6 days by MTT assay
Cytotoxicity against human CaSKi cells after 6 days by MTT assay
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[PMID: 10785428] |
| HT-3 | ED50 |
3.5 μg/mL
Compound: 8
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Cytotoxicity against human HT3 cells after 6 days by MTT assay
Cytotoxicity against human HT3 cells after 6 days by MTT assay
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[PMID: 10785428] |
| L1210 | IC50 |
62.5 μM
Compound: 148
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Cytotoxicity against mouse L1210 cells
Cytotoxicity against mouse L1210 cells
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[PMID: 23092389] |
| SiHa | ED50 |
5.5 μg/mL
Compound: 8
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Cytotoxicity against human SiHa cells after 6 days by MTT assay
Cytotoxicity against human SiHa cells after 6 days by MTT assay
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[PMID: 10785428] |
| T-24 | ED50 |
4.4 μg/mL
Compound: 8
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Cytotoxicity against human T24 cells after 6 days by MTT assay
Cytotoxicity against human T24 cells after 6 days by MTT assay
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[PMID: 10785428] |
体外実験
Trametenolic acid (1 mg/mL; 15-30 mins) induces the upregulation of autophagy-related proteins (Beclin-1, Atg12, and LC3) in B16-F1 cells by activating the ERK1/2 signaling pathway[1].
Trametenolic acid (1 mg/mL; 24 hours; B16-F1 cells) has dual synergic mechanisms to exert an anti-melanogenetic effect[1].
Trametenolic acid (0.1-4 mg/mL; 52 hours; B16-F1 cells) increase melanin synthesis by approximately 20% and 40% in cells transfected with siRNAs against mTOR and Atg5, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
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Cell Line:B16-F1 cells
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Concentration:1 mg/mL
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Incubation Time:15 and 30 mins
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Result:Increased the expression levels of phospho-p38, phospho-ERK1/2, Beclin-1, Atg12 and LC3 by approximately 46%, 10%, 48%, 126% and 49%, respectively.
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Cell Line:B16-F1 cells
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Concentration:1 mg/mL
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Incubation Time:24 hours
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Result:Increased the expression of ERK1/2 and p-ERK1/2 and decreased MITF, tyrosinase, and TRP 1 levels.
化学情報
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CAS 番号 24160-36-9
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性状 Solid
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分子量 456.70
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分子式 C30H48O3
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Color White to off-white
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SMILES
C[C@]([C@@]1(CC2)C)(CC[C@]1([H])[C@H](C(O)=O)CC/C=C(C)/C)C(CC[C@@]3([H])C4(C)C)=C2[C@]3(CC[C@@H]4O)C
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Structure Classification
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Initial Source
Laetiporus versisporus
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外:
DMSO : 50 mg/mL (109.48 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 25 mg/mL (54.74 mM); Suspended solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
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- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1896 mL | 10.9481 mL | 21.8962 mL | 54.7405 mL |
| 5 mM | 0.4379 mL | 2.1896 mL | 4.3792 mL | 10.9481 mL | |
| 10 mM | 0.2190 mL | 1.0948 mL | 2.1896 mL | 5.4741 mL | |
| 15 mM | 0.1460 mL | 0.7299 mL | 1.4597 mL | 3.6494 mL | |
| 20 mM | 0.1095 mL | 0.5474 mL | 1.0948 mL | 2.7370 mL | |
| 25 mM | 0.0876 mL | 0.4379 mL | 0.8758 mL | 2.1896 mL | |
| 30 mM | 0.0730 mL | 0.3649 mL | 0.7299 mL | 1.8247 mL | |
| 40 mM | 0.0547 mL | 0.2737 mL | 0.5474 mL | 1.3685 mL | |
| 50 mM | 0.0438 mL | 0.2190 mL | 0.4379 mL | 1.0948 mL | |
| 60 mM | 0.0365 mL | 0.1825 mL | 0.3649 mL | 0.9123 mL | |
| 80 mM | 0.0274 mL | 0.1369 mL | 0.2737 mL | 0.6843 mL | |
| 100 mM | 0.0219 mL | 0.1095 mL | 0.2190 mL | 0.5474 mL |
Trametenolic acid 関連分類
HY-N4169 関連分類
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