Trolox

Cat. No.: HY-101445G: Data Sheet Handling Instructions
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Trolox (GMP) is Trolox (HY-101445) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. Trolox is an analogue of vitamin E with a powerful antioxidant effect. Trolox is also a powerful inhibitor of membrane damage.

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Trolox Chemical Structure

CAS No. : 53188-07-1

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Other In-stock Forms of Trolox:

Trolox (Standard)

Other Forms of Trolox:

Trolox (Standard) In-stock

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	> 100 μM Compound: 2a	Antiproliferative activity against human A549 cells after 48 hrs by SRB assay Antiproliferative activity against human A549 cells after 48 hrs by SRB assay	[PMID: 29129514]
Erythrocyte	IC₅₀	101 μM Compound: trolox	Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs	[PMID: 12828473]
HBL-100	GI₅₀	> 100 μM Compound: 2a	Antiproliferative activity against human HBL100 cells after 48 hrs by SRB assay Antiproliferative activity against human HBL100 cells after 48 hrs by SRB assay	[PMID: 29129514]
HL-60	IC₅₀	0.3 μg/mL Compound: Trolox	Antioxidant activity in PMA-stimulated Homo sapiens (human) HL60 cells assessed as inhibition of intracellular ROS generation incubated for 30 min prior to PMA stimulation measured after 30 min by DCFH-DA assay Antioxidant activity in PMA-stimulated Homo sapiens (human) HL60 cells assessed as inhibition of intracellular ROS generation incubated for 30 min prior to PMA stimulation measured after 30 min by DCFH-DA assay	10.1007/s00044-011-9882-z
HL-60	IC₅₀	1.4 μM Compound: Trolox	Inhibition of PMA induced ROS generation in human HL60 cells assessed as inhibition of ROA mediated oxidation of DCFH Inhibition of PMA induced ROS generation in human HL60 cells assessed as inhibition of ROA mediated oxidation of DCFH	[PMID: 23547843]
HL-60	IC₅₀	3.6 μM Compound: trolox	Antioxidant activity in human HL60 cells assessed as inhibition of TPA-stimulated hydrogen peroxide induced DCFH-DA oxidation by fluorometric microplate assay Antioxidant activity in human HL60 cells assessed as inhibition of TPA-stimulated hydrogen peroxide induced DCFH-DA oxidation by fluorometric microplate assay	[PMID: 16562834]
HeLa	GI₅₀	> 100 μM Compound: 2a	Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay	[PMID: 29129514]
HepG2	IC₅₀	> 300 μM Compound: TroH	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
MCF7	IC₅₀	1461 μM Compound: 14	Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay	[PMID: 9873556]
MCF7	IC₅₀	> 300 μM Compound: TroH	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
MT4	IC₅₀	200 μM Compound: TroH	Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
Melan-a	IC₅₀	300 μM Compound: 2	Cytotoxicity against mouse Melan-a cells by modified crystal voilet assay Cytotoxicity against mouse Melan-a cells by modified crystal voilet assay	[PMID: 22071299]
Melan-a	IC₅₀	> 300 μM Compound: 2	Depigmentation activity in mouse Melan-a cells assessed as inhibition of melanin formation after 4 days Depigmentation activity in mouse Melan-a cells assessed as inhibition of melanin formation after 4 days	[PMID: 22071299]
NRK-52E	EC₅₀	1.9 μM Compound: Trolox	Cytoprotective activity against LPS-induced NRK-52E cells assessed as reduction in cell death measured every 5 min for 72 hrs by xCELLigence based RTCA analysis Cytoprotective activity against LPS-induced NRK-52E cells assessed as reduction in cell death measured every 5 min for 72 hrs by xCELLigence based RTCA analysis	[PMID: 32141747]
PC-12	IC₅₀	20 μM Compound: Trolox	Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced ROS production after 1 hr by DCFH-DA staining-based fluorometric analysis Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced ROS production after 1 hr by DCFH-DA staining-based fluorometric analysis	[PMID: 23751098]
PC-12	IC₅₀	20 μM Compound: Trolox	Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced reactive oxygen species formation after 1 hr by DCFH-DA dye-based fluorometric analysis Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced reactive oxygen species formation after 1 hr by DCFH-DA dye-based fluorometric analysis	[PMID: 26447940]
RAW264.7	IC₅₀	30 μM Compound: Trx	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced ROS production measured after 4 hrs by H2DCFDA-AM probe based fluorescence assay Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced ROS production measured after 4 hrs by H2DCFDA-AM probe based fluorescence assay	[PMID: 29275229]
RPMI-8226	IC₅₀	> 300 μM Compound: TroH	Cytotoxicity against human RPMI8226 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human RPMI8226 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
SH-SY5Y	IC₅₀	49.55 μM Compound: Trolox	Antioxidant activity against tert-butyl hydroperoxide-induced ROS evoked cell death in human SH-SY5Y cells Antioxidant activity against tert-butyl hydroperoxide-induced ROS evoked cell death in human SH-SY5Y cells	[PMID: 18605718]
SH-SY5Y	IC₅₀	82.3 μM Compound: Trolox	Antioxidant activity in human SH-SY5Y cells assessed as inhibition of H2O2-induced DCFH oxidation measured after 1 hr by DCFH-DA dye based fluorescence assay Antioxidant activity in human SH-SY5Y cells assessed as inhibition of H2O2-induced DCFH oxidation measured after 1 hr by DCFH-DA dye based fluorescence assay	[PMID: 27541262]
SW1573	GI₅₀	> 100 μM Compound: 2a	Antiproliferative activity against human SW1573 cells after 48 hrs by SRB assay Antiproliferative activity against human SW1573 cells after 48 hrs by SRB assay	[PMID: 29129514]
T47D	GI₅₀	> 100 μM Compound: 2a	Antiproliferative activity against human T47D cells after 48 hrs by SRB assay Antiproliferative activity against human T47D cells after 48 hrs by SRB assay	[PMID: 29129514]
U-937	IC₅₀	300 μM Compound: TroH	Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
WiDr	GI₅₀	> 100 μM Compound: 2a	Antiproliferative activity against human WiDr cells after 48 hrs by SRB assay Antiproliferative activity against human WiDr cells after 48 hrs by SRB assay	[PMID: 29129514]

In Vitro

Trolox (GMP) has shown to protect mammalian cells from oxidative damage. Trolox (GMP) is effective in preventing myocyte necrosis in cell culture studies and in a canine model of two hours of left anterior descending coronary artery (LAD) occlusion followed by four hours of reperfusion^[1].
Trolox (GMP) could prevent oxidative stress-induced apoptosis in thymocytes. Pre- or post-treatment of cells with Trolox (GMP) reduced H₂O₂-induced DNA fragmentation to control levels and below. ^[2].
Trolox (GMP) is a hydrophilic analog of alpha-tocopherol and reported to scavenge peroxyl radicals better than vitamin E in sodium dodecyl sulfate micelles and in liposomes. Trolox (GMP) prolongs substantially the survival of human ventricular myocytes and hepatocyte against oxyradicals generated with xanthine oxidase plus hypoxanthine, and prevented lysis of red cells exposed to an azo-initiator^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

250.29

Formula

C₁₄H₁₈O₄

CAS No.

53188-07-1

SMILES

O=C(C1(C)CCC2=C(C)C(O)=C(C)C(C)=C2O1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Mickle DA, et al. Myocardial salvage with trolox and ascorbic acid for an acute evolving infarction. Ann Thorac Surg. 1989 Apr;47(4):553-7. [Content Brief]

[2]. Forrest VJ, et al. Oxidative stress-induced apoptosis prevented by Trolox. Free Radic Biol Med. 1994 Jun;16(6):675-84. [Content Brief]

[3]. Wu TW, et al. The cytoprotective effect of Trolox demonstrated with three types of human cells. Biochem Cell Biol. 1990 Oct;68(10):1189-94. [Content Brief]

Trolox Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Trolox (GMP)53188-07-1Reactive Oxygen Species (ROS)FerroptosisApoptosisAntioxidantGMPInhibitorinhibitorinhibit

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