Vari Fluor 647-Streptavidin
Based on 1 Customer Validation
Vari Fluor 647-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=650 nm/665 nm.
Product Concentration: 0.8 mg/mL
For research use only. We do not sell to patients.
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Product Concentration: 0.8 mg/mL
General Protocol
1.Protein treatment
Before use, centrifuge at 5000× g for 3 min, only the supernatant is used for experiments to eliminate protein aggregates and reduce non-specific background staining.
2.Labeling
Dilute Vari Fluor-Streptavidin at 1:500-1:1000, which can be adjusted according to the specific conditions of the protein or antibody.
Storage
-20°C,
Protect from light
Precautions
1. The actual content of the dye is small, dissolve it directly in the tube after receiving it for experiments.
2. Before use, please centrifuge the product to the bottom of the tube instantaneously before subsequent experiments.
3. This product is intended for scientific research only by professionals and is not to be used for clinical diagnosis or treatment, food or medicine.
4. For your safety and health, please wear lab coat and disposable gloves.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
665
650
Chemical Information
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Appearance Liquid
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Color Light blue to blue
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SMILES
[Vari Fluor 647-Streptavidin]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (259 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)