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  3. Vari Fluor 680-Streptavidin

Vari Fluor 680-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=680 nm/701 nm.

For research use only. We do not sell to patients.

Vari Fluor 680-Streptavidin

Vari Fluor 680-Streptavidin Chemical Structure

Size Price Stock Quantity
Solvent
50 μL USD 30 In-stock
Solvent
500 μL USD 240 In-stock
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

Vari Fluor 680-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=680 nm/701 nm.

In Vitro

General Protocol
1.Protein treatment
Before use, centrifuge at 5000× g for 3 min, only the supernatant is used for experiments to eliminate protein aggregates and reduce non-specific background staining.
2.Labeling
Dilute Vari Fluor-Streptavidin at 1:500-1:1000, which can be adjusted according to the specific conditions of the protein or antibody.

Storage
-20°C,
Protect from light

Precautions
1. The actual content of the dye is small, dissolve it directly in the tube after receiving it for experiments.
2. Before use, please centrifuge the product to the bottom of the tube instantaneously before subsequent experiments.
3. This product is intended for scientific research only by professionals and is not to be used for clinical diagnosis or treatment, food or medicine.
4. For your safety and health, please wear lab coat and disposable gloves.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Liquid

Color

Light blue to blue

Emission (Em)

701

Excitation (Ex)

680

SMILES

[Vari Fluor 680-Streptavidin]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

References
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Vari Fluor 680-Streptavidin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Vari Fluor 680-Streptavidin
Cat. No.:
HY-D1804
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