Verrucarin A
Based on 1 publication(s) in Google Scholar
Verrucarin A (Muconomycin A), a Type D macrocyclic mycotoxin derived from the pathogen fungus Myrothecium verrucaria, is an inhibitor of protein synthesis. Verrucarin A inhibits growth of leukemia cell lines and activates caspases and apoptosis and inflammatory signaling in macrophages. Verrucarin A effectively increased the phosphorylation of p38 MAPK and diminished the phosphorylation of ERK/Akt. Verrucarin A caused cell cycle deregulation through the induction of p21 and p53.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 3148-09-2
- Formula: C27H34O9
- Molecular Weight:502.55
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Verrucarin A
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
0.2 ng/mL
Compound: 3
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Cytotoxicity against human HL60 cells after 72 hrs by WST8 assay
Cytotoxicity against human HL60 cells after 72 hrs by WST8 assay
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[PMID: 11277768] |
| K562 | IC50 |
0.003 μM
Compound: 14
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Cytotoxicity against human K562 cells by disk diffusion soft agar colony formation assay
Cytotoxicity against human K562 cells by disk diffusion soft agar colony formation assay
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[PMID: 31117520] |
| L1210 | IC50 |
0.35 ng/mL
Compound: 3
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Cytotoxicity against mouse L1210 cells after 72 hrs by WST8 assay
Cytotoxicity against mouse L1210 cells after 72 hrs by WST8 assay
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[PMID: 11277768] |
| SW 1116 | IC50 |
0.0018 μM
Compound: 14
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Cytotoxicity against human SW1116 cells by disk diffusion soft agar colony formation assay
Cytotoxicity against human SW1116 cells by disk diffusion soft agar colony formation assay
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[PMID: 31117520] |
Verrucarin A (0-0.6 μM/ml; 24-48 hours)-induces time- and dose-dependent growth inhibition in MCF-7 cells[1].
Verrucarin A increases the levels of reactive oxygen species (ROS), and subsequently induces mitochondrial membrane potential (Δψm) loss, leading to the increase of Bax/Bcl-2 ratio, cytochrome c release, caspase activation, PARP degradation, and apoptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 cells
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Concentration:0-0.6 μM/ml
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Incubation Time:24 and 48 hours
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Result:Growth of MCF-7 cells is significantly inhibited in a dose- and time-dependent manner, with the IC50s of 0.41 and 0.29 μM/ml for 24- and 48-h treatment periods, respectively.
Chemical Information
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CAS No. 3148-09-2
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Appearance Solid
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Molecular Weight 502.55
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Formula C27H34O9
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Color White to light yellow
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SMILES
C[C@@]12[C@]3(CO3)[C@@]4([H])C[C@@]1([H])OC(/C=C/C=C/C(OCC[C@@H](C)[C@H](O)C(OC[C@]25[C@](C=C(C)CC5)([H])O4)=O)=O)=O
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Synonyms
Muconomycin A
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 10 mg/mL (19.90 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Palanivel K, et al. Verrucarin A alters cell-cycle regulatory proteins and induces apoptosis through reactive oxygen species-dependent p38MAPK activation in the human breast cancer cell line MCF-7. Tumour Biol. 2014;35(10):10159-10167. [Content Brief]
[2]. Palanivel K, et al. Verrucarin A, a protein synthesis inhibitor, induces growth inhibition and apoptosis in breast cancer cell lines MDA-MB-231 and T47D. Biotechnol Lett. 2013;35(9):1395-1403. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9899 mL | 9.9493 mL | 19.8985 mL | 49.7463 mL |
| 5 mM | 0.3980 mL | 1.9899 mL | 3.9797 mL | 9.9493 mL | |
| 10 mM | 0.1990 mL | 0.9949 mL | 1.9899 mL | 4.9746 mL | |
| 15 mM | 0.1327 mL | 0.6633 mL | 1.3266 mL | 3.3164 mL |
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