2. PROTAC
  3. Ligands for E3 Ligase
  4. VH032

VH032 is a VHL ligand used in the recruitment of the von Hippel-Lindau (VHL) protein. VH032 is a VHL/HIF-1α interaction inhibitor with a KdPROTACs.

For research use only. We do not sell to patients.

CAS No. : 1448188-62-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of VH032:

VH032 Related Antibodies

Top Publications Citing Use of Products

    VH032 purchased from MedChemExpress. Usage Cited in: J Control Release. 2026 May 10:393:114828.

    Western blot analysis of BRD4 levels in HeLa cells pretreated with JQ-1 for 6 h or VH032 (1 μM) for 6 h, followed by treatment with HBPROTAC [Tz-PU (2 h pretreatment) + TCO-MZ1] for 8 h.

    VH032 purchased from MedChemExpress. Usage Cited in: J Control Release. 2026 May 10:393:114828.

    Western blot analysis of Bcl-xL levels in HeLa cells pretreated with MG-132, ABT-263 or VH032 (1 μM) for 6 h, followed by treatment with HBPROTAC [Tz-PU (2 h pretreatment) + TCO-DT2216] for 8 h.

    VH032 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Sep 18:44:102254.  [Abstract]

    VH032 (0-100 μm; 24 h) inhibits the proliferation of U87MG and U251 cells.

    VH032 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Sep 18:44:102254.  [Abstract]

    VH032 inhibits the migration and invasion of glioma cells. a. VH032 inhibits the migration of glioma cells in wound healing assay. The migratory capacity of U87MG (treated with 59.2 μM VH032) and U251 (treated with 85.07 μM VH032) cells was evaluated at 0 h and 24 h.

    VH032 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Sep 18:44:102254.  [Abstract]

    Transwell assesses were performed using U87MG (59.2 μM VH032) and U251 (85.07 μM VH032) to evaluate the effect of VH032 on glioma cell invasion.

    VH032 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Sep 18:44:102254.  [Abstract]

    VH032 induced the apoptosis of glioma cells. Flow cytometry analysis of apoptotic U87MG (59.2 μM VH032) and U251 (85.07 μM VH032) cells via Annexin V-FITC/PI staining after 24 h of treatment. The effect of VH032 on the apoptosis of glioma cells was significantly increased.

    VH032 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Sep 18:44:102254.  [Abstract]

    VH032 induced G2/M cell cycle arrest in glioma cells. Flow cytometry analysis of cell cycle distribution in U87MG (59.2 μM VH032) and U251 (85.07 μM VH032) cells after 24 h of treatment, and the proportion of cells in the G2/M phase increased significantly

    VH032 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Sep 18:44:102254.  [Abstract]

    VH032 affects cell viability, migration, invasion and apoptosis by inhibiting the VHL/HIF-1α/VEGF signalling pathway. Western blotting analysis of VHL, HIF-1α, and VEGF protein levels in U87MG (59.2 μM VH032) and U251 (85.07 μM VH032) cells after 24 h of treatment.

    VH032 purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2022 May 30;721:109194.  [Abstract]

    Western blotting analysis of AR in BT549 cells treated with AR-PROTAC (100 nM), or co-treated with enzalutamide (1 μM), VH032 (1 μM), MLN924 (1 μM), or MG132 (10 μM) for 24 h.

    View All Ligands for E3 Ligase Isoform Specific Products:

    View All Isoforms
    von Hippel-Lindau (VHL) MDM2 Cereblon IAP KLHDC2 Fbxo3 RNF4 DCAF11 DCAF1 UHRF1 TRIM21 NEURL1B

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    VH032 is a VHL ligand used in the recruitment of the von Hippel-Lindau (VHL) protein. VH032 is a VHL/HIF-1α interaction inhibitor with a KdPROTACs[1][2][3].

    IC50 & Target[1]

    VHL

     

    In Vitro

    PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    VH032 Related Antibodies
    Molecular Weight

    472.60

    Formula

    C24H32N4O4S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC(N=CS1)=C1C2=CC=C(CNC([C@@H]3C[C@@H](O)CN3C([C@H](C(C)(C)C)NC(C)=O)=O)=O)C=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (211.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1160 mL 10.5798 mL 21.1595 mL
    5 mM 0.4232 mL 2.1160 mL 4.2319 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 3.5 mg/mL (7.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3.5 mg/mL (7.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1160 mL 10.5798 mL 21.1595 mL 52.8989 mL
    5 mM 0.4232 mL 2.1160 mL 4.2319 mL 10.5798 mL
    10 mM 0.2116 mL 1.0580 mL 2.1160 mL 5.2899 mL
    15 mM 0.1411 mL 0.7053 mL 1.4106 mL 3.5266 mL
    20 mM 0.1058 mL 0.5290 mL 1.0580 mL 2.6449 mL
    25 mM 0.0846 mL 0.4232 mL 0.8464 mL 2.1160 mL
    30 mM 0.0705 mL 0.3527 mL 0.7053 mL 1.7633 mL
    40 mM 0.0529 mL 0.2645 mL 0.5290 mL 1.3225 mL
    50 mM 0.0423 mL 0.2116 mL 0.4232 mL 1.0580 mL
    60 mM 0.0353 mL 0.1763 mL 0.3527 mL 0.8816 mL
    80 mM 0.0264 mL 0.1322 mL 0.2645 mL 0.6612 mL
    100 mM 0.0212 mL 0.1058 mL 0.2116 mL 0.5290 mL
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    VH032 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    VH0321448188-62-2VH 032VH-032Ligands for E3 LigaseE3 ligase-recruiting MoietyVHLligandHIF-1αPROTACInhibitorinhibitorinhibit

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