WX-293
WX-293 is a highly selective urokinase-type plasminogen activator (uPA) inhibitor with a Ki value of 2.4 μM. WX-293 abolishes hypoxia-induced keratinocyte migration and in vitro wound closure. WX-293 can be used in research on hypoxia-mediated skin wounds and solid malignant tumors.
For research use only. We do not sell to patients.
- CAS No.: 282718-42-7
- Formula: C19H27N5O
- Molecular Weight:341.45
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
WX-293 (0.1-0.2 μM; 16 h) inhibits hypoxia-induced in vitro wound healing of PAM 212 mouse keratinocytes in a concentration-dependent manner[1].
WX-293 potently and selectively inhibits purified human urokinase-type plasminogen activator (uPA) with a Ki value of 2.4 µM, and its mechanism of action involves targeting the S1′ pocket of uPA via a non-traditional binding mode[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:PAM 212 murine keratinocytes
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Concentration:0.1 μM, 0.2 μM
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Incubation Time:16 h
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Result:Inhibited hypoxia-enhanced wound closure by 70% (0.1 μM).
Inhibited uPA activity by 55.3% (0.1 μM).
Completely abolished hypoxia-enhanced wound closure (0.2 μM).
Inhibited uPA activity by 74.0% (0.2 μM).
Exhibited no effect on wound closure in normoxic cultures at either concentration.
Chemical Information
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CAS No. 282718-42-7
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Molecular Weight 341.45
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Formula C19H27N5O
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SMILES
O=C(NC12CC3CC(C2)CC(C1)C3)NCC4=CC=C(NC(N)=N)C=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Scratch/Wound-Healing Migration Assay
The scratch/wound-healing migration assay measures collective migration of adherent cells into an experimentally created cell-free gap in a confluent monolayer. The readout is generated by imaging the gap immediately after scratching and at later time points, then quantifying reduction in wound area, wound width, or percentage closure as cells move into the denuded region. Gap closure reflects cell migration but may also include cell proliferation, so interpretation should distinguish migration-focused conditions from proliferation-driven closure when possible, such as by using short assay windows, serum-controlled conditions, cell counting, or proliferation controls reported in published protocols.
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Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
Purity & Documentation
References
[1]. Daniel RJ, et al. Increased migration of murine keratinocytes under hypoxia is mediated by induction of urokinase plasminogen activator. J Invest Dermatol. 2002;119(6):1304-1309. [Content Brief]
[2]. Muehlenweg B, et al. Interference with the urokinase plasminogen activator system: a promising therapy concept for solid tumours. Expert Opin Biol Ther. 2001;1(4):683-691. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
WX-293 Related Classifications
HY-182450 Related Classifications
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