ZDZ-553

Cat. No.: HY-181792 Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
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ZDZ-553 is an orally active STAT1 inhibitor with an IC₅₀ value of 0.87 μM. ZDZ-553 modulates STAT1 signaling to affect downstream lipid metabolism and inflammatory pathways. ZDZ-553 attenuates hepatic steatosis in NASH mouse models. ZDZ-553 reduces inflammatory responses in NASH mouse models. ZDZ-553 can be used for the research of nonalcoholic steatohepatitis.

For research use only. We do not sell to patients.

ZDZ-553 Chemical Structure

Size	Price	Stock
5 mg		Ask For Quote & Lead Time
10 mg		Ask For Quote & Lead Time
25 mg		Ask For Quote & Lead Time
50 mg		Ask For Quote & Lead Time
100 mg		Ask For Quote & Lead Time

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

STAT1

0.87 μM (IC₅₀)

In Vitro

ZDZ-553 (24 h) potently inhibits STAT1 activity in stable 293T-STAT1/GAS-Luc cells with an IC₅₀ of 0.87 μM and shows no cytotoxicity at concentrations up to 200 μM^[1].
ZDZ-553 (0.052 μM; 0, 30, 60, 120 min) has excellent metabolic stability in human, mouse, and rat liver microsomes, with a T_1/2 >120 min across all species^[1].
ZDZ-553 (0-200 μM; 24 h) exhibits no cytotoxicity in HepG2 cells at concentrations up to 200 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	HepG2 cells
Concentration:	0-200 μM (serial 2-fold dilutions starting at 200 μM)
Incubation Time:	24 h
Result:	Showed no cytotoxicity after 24 h of exposure, with no decrease in cell viability even at the highest tested concentration of 200 μM.

Parmacokinetics

Species	Dose	Route	AUC_0-inf	C_max	T_1/2	MRT_0-inf	T_max	F
Rat^[1]	30 mg/kg	i.v.	69,512 ng·h/mL	2467 ng/mL	16.63 h	24.3 h	/	/
Rat^[1]	30 mg/kg	p.o.	12,599 ng·h/mL	819 ng/mL	12.18 h	18.8 h	1.13 h	18.1 %

In Vivo

ZDZ-553 (30-60 mg/kg; p.o.; daily; 4 weeks) administered orally at 30 and 60 mg/kg for 4 weeks dose-dependently ameliorates hepatic steatosis, liver damage, and inflammation in HF-CDAA-induced NASH mice, with the 60 mg/kg dose showing greater efficacy than the 30 mg/kg dose and the positive control Dios^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J (male, 7−8 weeks old, 20-23 g, HF-CDAA diet-induced)^[1]
Dosage:	30 mg/kg; 60 mg/kg
Administration:	p.o.; daily; 4 weeks
Result:	Reduced hepatic triglyceride (TG) and total cholesterol (TC) levels, reduced serum ALT and AST levels; reduced mRNA expression of hepatic acetyl-CoA carboxylase (ACC), fatty acid synthase (FASN), FATP-1, IL-6, IL-1β, TNF-α, and CXCL10; decreased NAFLD Activity Score (NAS) compared to vehicle controls; reduced hepatocellular ballooning (H&E staining) and reduced size/quantity of lipid droplets (Oil Red O staining) at 30 mg/kg. Produced greater dose-dependent reductions in hepatic TG, TC, serum AST, and mRNA expression of ACC, FASN, FATP-1, IL-6, IL-1β, TNF-α, and CXCL10 compared to the 30 mg/kg dose; resulted in a larger decrease in NAS compared to the 30 mg/kg dose; showed more pronounced improvements in hepatocellular ballooning and lipid droplet accumulation (histological staining) compared to the 30 mg/kg dose at 60 mg/kg. Showed more significant reductions in hepatic TG, TC, serum AST, and inflammatory cytokine mRNA expression than the positive control Dios at 60 mg/kg at both doses.

Molecular Weight

404.26

Formula

C₁₈H₁₀F₆O₄

Appearance

Solid

Color

Off-white to light yellow

SMILES

COC1=C2C(C=C(OC2=CC(O)=C1)C3=CC(C(F)(F)F)=CC(C(F)(F)F)=C3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (206.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4737 mL	12.3683 mL	24.7366 mL
5 mM	0.4947 mL	2.4737 mL	4.9473 mL
10 mM	0.2474 mL	1.2368 mL	2.4737 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (249 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang Z, et al. Design, Synthesis, and Biological Evaluation of Chromone Derivatives as STAT1 Inhibitors for Treatment of Nonalcoholic Steatohepatitis. J Med Chem. 2026 Feb 12;69(3):2509-2538. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4737 mL	12.3683 mL	24.7366 mL	61.8414 mL
	5 mM	0.4947 mL	2.4737 mL	4.9473 mL	12.3683 mL
	10 mM	0.2474 mL	1.2368 mL	2.4737 mL	6.1841 mL
	15 mM	0.1649 mL	0.8246 mL	1.6491 mL	4.1228 mL
	20 mM	0.1237 mL	0.6184 mL	1.2368 mL	3.0921 mL
	25 mM	0.0989 mL	0.4947 mL	0.9895 mL	2.4737 mL
	30 mM	0.0825 mL	0.4123 mL	0.8246 mL	2.0614 mL
	40 mM	0.0618 mL	0.3092 mL	0.6184 mL	1.5460 mL
	50 mM	0.0495 mL	0.2474 mL	0.4947 mL	1.2368 mL
	60 mM	0.0412 mL	0.2061 mL	0.4123 mL	1.0307 mL
	80 mM	0.0309 mL	0.1546 mL	0.3092 mL	0.7730 mL
	100 mM	0.0247 mL	0.1237 mL	0.2474 mL	0.6184 mL

ZDZ-553 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ZDZ-553ZDZ553ZDZ 553STATrat liver microsomeslipid metabolismNASH mouse modelsmouse liver microsomeshuman liver microsomesSTAT1hepatic steatosisHepG2 cellsinflammatory pathways293T-STAT1/GAS-Luc cellsInhibitorinhibitorinhibit

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