α-Terthienylmethanol
α-Terthienylmethanol is a terthiophene isolated from the n-hexane fraction of E. prostrata. α-Terthienylmethanol has potent cytotoxic activity against human endometrial cancer cells (Hec1A and Ishikawa) (IC50 < 1 μM). α-Terthienylmethanol increases the intracellular level of ROS and decreases that of GSH.
For research use only. We do not sell to patients.
- CAS No.: 13059-93-3
- Formula: C13H10OS3
- Molecular Weight:278.41
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| Sf9 | IC50 |
4.0 x 10-6M
Compound: 7
|
Inhibition of protein kinase C(1:1mixture of PKC alpha & beta) expressed in Sf9 insect cells
Inhibition of protein kinase C(1:1mixture of PKC alpha & beta) expressed in Sf9 insect cells
|
[PMID: 9873605] |
In Vitro
Trithiophene is a conjugated organosulfur heterocyclic compound consisting of three thiophene five-membered heterocycles linked sequentially through α-carbon sites. It possesses an extended π-electron conjugation system and excellent photoelectric properties (such as fluorescence emission and conductivity). It is naturally found in the metabolites of certain marine plants and microorganisms, and often exhibits various biological activities such as antibacterial, antitumor, and antioxidant effects.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 13059-93-3
-
Molecular Weight 278.41
-
Formula C13H10OS3
-
SMILES
OCC1=CC=C(C2=CC=C(C3=CC=CS3)S2)S1
-
Structure Classification
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
α-Terthienylmethanol Related Classifications
HY-N2852 Related Classifications
Powered by Bioz