α1-3,4 Fucosidase, Bifidobacterium bifidum
Based on 1 Customer Validation
α1-3,4 Fucosidase, Bifidobacterium bifidum is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing α1-3 and α1-4 linked fucose residues from oligosaccharides and glycoproteins.
For research use only. We do not sell to patients.
- CAS No.: 9037-65-4
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
IC50 & Target
others
In Vitro
Product Information This product is recombinantly expressed in E. coli, with a C-terminal His-tag, a molecular weight of 160 kDa, and a buffer composition of 20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% glycerol. It can catalyze the hydrolysis of α1-3 and α1-4-linked fucose residues at the terminals of oligosaccharides and glycoproteins. Instructions 1. Add substrate, 50 mM sodium acetate, pH 5.5, 5 mM CaCl2 to the reaction system, mix well, and add appropriate amount of enzyme. 2. Incubate the sample at 37°C for 2-4h or overnight. Notes When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed shaking.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.2.1.51
Specific Activity
>2000 U/mL
Unit Definition
One unit is defined as the amount of enzyme that hydrolyze 1 μmol Galβ1, 4(FUCα1,3) to Galβ1,4Glc and Fuc at 37°C, pH 6.0 with the 10 μL of total reaction volume.
Chemical Information
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CAS No. 9037-65-4
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[a1-3,4 Fucosidase]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)