2. Neuronal Signaling
  3. Amyloid-β
  4. β-Amyloid (25-35), HFIP-treated

β-Amyloid (25-35), HFIP-treated  (Synonyms: Amyloid beta-peptide (25-35), HFIP-treated; Aβ25-35, HFIP-treated; β-Amyloid peptide (25-35), HFIP-treated)

Cat. No.: HY-P0128A Purity: 98.18%
COA
SDS
Handling Instructions Technical Support

β-Amyloid (25-35) (Amyloid beta-peptide (25-35)), HFIP-treated is a β-Amyloid (25-35) (HY-P0128) treated with HFIP. β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide, has shown neurotoxic activities in cultured cells.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 131602-53-4

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1 mg In-stock
5 mg (1 mg * 5) In-stock
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Customer Review

Based on 42 publication(s) in Google Scholar

Other Forms of β-Amyloid (25-35), HFIP-treated:

β-Amyloid (25-35), HFIP-treated Related Antibodies

Top Publications Citing Use of Products

42 Publications Citing Use of MCE β-Amyloid (25-35), HFIP-treated

Cell Imaging/Staining
Flow Cytometry
ELISA
WB
IF
Bio/Physico-chemical Assay
Apoptosis Analysis

    β-Amyloid (25-35), HFIP-treated purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642.  [Abstract]

    DCFH-DA probe fluorescence Images of different groups of BV2 cells induced by β-Amyloid (25-35)(Aβ; 10µM; 24 h) , scale bar =250 μm.

    β-Amyloid (25-35), HFIP-treated purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642.  [Abstract]

    Flow cytometry detection of ROS in different groups including β-Amyloid (25-35)(Aβ; 10µM; 24 h) of BV2 cells.

    β-Amyloid (25-35), HFIP-treated purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642.  [Abstract]

    Quantitative analysis of ELISA results for IL-1β, IL-6, TNF-α, and IL-10 in different groups including β-Amyloid (25-35)(Aβ; 10µM; 24 h) of BV2 cells.

    β-Amyloid (25-35), HFIP-treated purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642.  [Abstract]

    Western blot analysis of IL-1β, IL-6, TNF-α, and IL-10 related pathological proteins in different groups including β-Amyloid (25-35)(Aβ; 10µM; 24 h) of BV2 cells.

    β-Amyloid (25-35), HFIP-treated purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642.  [Abstract]

    Fluorescence images of IL-1β (I) and IL-6 (J) in different groups including β-Amyloid (25-35)(Aβ; 10µM; 24 h) of BV2 cells, scale bar = 50 μm.

    β-Amyloid (25-35), HFIP-treated purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642.  [Abstract]

    Quantitative analysis of fluorescence curves for Thioflavin T (THT) detection of Aβ fiber aggregation in different groups including induction by β-Amyloid (25-35) (Aβ; 20 µM; 0-72 h) of BV2 cells.

    β-Amyloid (25-35), HFIP-treated purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642.  [Abstract]

    Image of Annexin V-FITC apoptosis detection in different groups including β-Amyloid (25-35) (Aβ; 10 µM; 24 h) of SH-SY5Y cells.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    β-Amyloid (25-35) (Amyloid beta-peptide (25-35)), HFIP-treated is a β-Amyloid (25-35) (HY-P0128) treated with HFIP. β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide, has shown neurotoxic activities in cultured cells[1].

    In Vitro

    The amino acid sequence of Aβ(25-35) peptide is NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy[1].
    Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events[2].
    β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. The resulting film is dissolved in anhydrous DMSO at 5 mM and then dilutes into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.
    2. Next, the solution is aged for 7 days at 37°C. The sample is then centrifuged at 14000g for 10 min at 4-8 °C; the soluble oligomers are in the supernatant. The supernatant is diluted 10-200-fold for experiments.
    Methods vary depends on the downstream applications.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    β-Amyloid (25-35), HFIP-treated Related Antibodies
    Molecular Weight

    1060.27

    Formula

    C45H81N13O14S
    CAS No.
    Appearance

    Solid-Liquid Mixture

    Color

    Colorless to off-white

    Sequence

    Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met
    Sequence Shortening

    GSNKGAIIGLM
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Pure form -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (9.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9432 mL 4.7158 mL 9.4316 mL
    5 mM 0.1886 mL 0.9432 mL 1.8863 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Concentration (final)

    C2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 98.18%

    SDS (252 KB)

    COA (246 KB) RP-HPLC (266 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    Cell viability is determined by a modified MTS assay, which is based on the conversion of Tetrazolium salt by mitochondrial dehydrogenase to a formazan product spectrophotometrically measurable at 490 nm. PC12 cells are plated in 96-well plates at a density of 10 000 cells/well and maintained for 16 h in complete medium. Cells are then incubated in the absence (control) and presence of 40 μM Aβ(31-35) and Aβ(25-35) with reduced, oxidized and norleucine-substituted methionine-35 staurosporine 10 μM is used as positive control of 100% of cellular death. After 48 h of peptide-incubation, 20 μL of MTS reagent (2.0 mg/mL) is added to each well. The cells are then incubated for 30-45 min at 37 °C in a 5% CO2 incubator. The reaction is stopped by adding 25 μL of 10% SDS. The plates are read with a microplate reader at 490 nm. Each data point is obtained using a triplet-well assay[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.9432 mL 4.7158 mL 9.4316 mL 23.5789 mL
    5 mM 0.1886 mL 0.9432 mL 1.8863 mL 4.7158 mL
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    β-Amyloid (25-35), HFIP-treated Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    β-Amyloid (25-35), HFIP-treated131602-53-4Amyloid beta-peptide (25-35), HFIP-treated Aβ25-35, HFIP-treatedβ-Amyloid peptide (25-35), HFIP-treatedAmyloid-ββ-amyloid peptideAβAbetaInhibitorinhibitorinhibit

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