1-Methylcytosine
Based on 1 Customer Validation
1-Methylcytosine is a pyrimidinone that serves as a nucleobase for hachimoji DNA and pairs with isoguanine. 1-Methylcytosine exerts weak antiproliferative activity against cervical cancer cells. 1-Methylcytosine can be used in the research of cervical cancer.
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- Pureza : 99.01%
- No. CAS: 1122-47-0
- Fòrmula: C5H7N3O
- Peso molecular:125.13
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
12 μM
Compound: 21
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Growth inhibition of human HeLa cells after 72 hrs by MTT assay
Growth inhibition of human HeLa cells after 72 hrs by MTT assay
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[PMID: 18640746] |
| MCF7 | IC50 |
>100 μM
Compound: 21
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Growth inhibition of human MCF7 cells after 72 hrs by MTT assay
Growth inhibition of human MCF7 cells after 72 hrs by MTT assay
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[PMID: 18640746] |
| MIA PaCa-2 | IC50 |
>100 μM
Compound: 21
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Growth inhibition of human MIAPaCa2 cells after 72 hrs by MTT assay
Growth inhibition of human MIAPaCa2 cells after 72 hrs by MTT assay
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[PMID: 18640746] |
| NCI-H460 | IC50 |
>100 μM
Compound: 21
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Growth inhibition of human H460 cells after 72 hrs by MTT assay
Growth inhibition of human H460 cells after 72 hrs by MTT assay
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[PMID: 18640746] |
| SW-620 | IC50 |
>100 μM
Compound: 21
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Growth inhibition of human SW620 cells after 72 hrs by MTT assay
Growth inhibition of human SW620 cells after 72 hrs by MTT assay
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[PMID: 18640746] |
In Vitro
1-Methylcytosine (Compound 21) (10-8-10-4 M; 72 h) weakly inhibits the proliferation of human cervical cancer HeLa cells, with an IC50 value of 12 μM, and shows no antiproliferative activity against human tumor cell lines MiaPaCa-2, SW620, MCF-7 and H460[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:human cervical carcinoma HeLa, human pancreatic carcinoma MiaPaCa-2, human colon carcinoma SW620, human breast carcinoma MCF-7, human lung carcinoma H460
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Concentration:10-8-10-4 M
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Incubation Time:72 h
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Result:Exhibited weak antiproliferative activity on HeLa cell line, with an IC50 value of 12 μM.
Showed no growth inhibitory effect on MiaPaCa-2, SW620, MCF-7, or H460 cell lines (IC50 >100 μM for all).
Chemical Information
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No. CAS 1122-47-0
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Appearance Solid
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Peso molecular 125.13
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Fòrmula C5H7N3O
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Color White to off-white
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SMILES
NC(C=CN1C)=NC1=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
In Vitro:
DMSO : 10 mg/mL (79.92 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocolo
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
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Ficha de datos (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Noll S, et al. Synthesis of modified pyrimidine bases and positive impact of chemically reactive substituents on their in vitro antiproliferative activity. Eur J Med Chem. 2009;44(3):1172-1179. [Content Brief]
[2]. Ruiz J, et al. New palladium(II) and platinum(II) complexes with the model nucleobase 1-methylcytosine: antitumor activity and interactions with DNA. Inorg Chem. 2005;44(21):7365-7376. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.9917 mL | 39.9584 mL | 79.9169 mL | 199.7922 mL |
| 5 mM | 1.5983 mL | 7.9917 mL | 15.9834 mL | 39.9584 mL | |
| 10 mM | 0.7992 mL | 3.9958 mL | 7.9917 mL | 19.9792 mL | |
| 15 mM | 0.5328 mL | 2.6639 mL | 5.3278 mL | 13.3195 mL | |
| 20 mM | 0.3996 mL | 1.9979 mL | 3.9958 mL | 9.9896 mL | |
| 25 mM | 0.3197 mL | 1.5983 mL | 3.1967 mL | 7.9917 mL | |
| 30 mM | 0.2664 mL | 1.3319 mL | 2.6639 mL | 6.6597 mL | |
| 40 mM | 0.1998 mL | 0.9990 mL | 1.9979 mL | 4.9948 mL | |
| 50 mM | 0.1598 mL | 0.7992 mL | 1.5983 mL | 3.9958 mL | |
| 60 mM | 0.1332 mL | 0.6660 mL | 1.3319 mL | 3.3299 mL |
1-Methylcytosine Related Classifications
HY-W006395 Related Classifications
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