1-beta-D-Arabinofuranosyluracil
Based on 1 publication(s) in Google Scholar
1-beta-D-Arabinofuranosyluracil (Uracil 1-β-D-arabinofuranoside) isolated from the Caribbean sponge Tectitethya crypta, is a methoxyadenosine derivative. 1-beta-D-Arabinofuranosyluracil has demonstrated a diverse bioactivity profile including anti-inflammatory activity, analgesic and vasodilation properties. 1-beta-D-Arabinofuranosyluracil reduces a proliferation of mouse lymphoma cells.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 3083-77-0
- Formula: C9H12N2O6
- Molecular Weight:244.20
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-N6652R
1-beta-D-Arabinofuranosyluracil (Standard)Anti-Inflammatory Agent -
HY-107856
5-FluorouridineApoptosis Inducer -
HY-13605
CytarabineDNA Synthesis Inhibitor -
HY-A0061
TrifluridineAnti-Cancer Agent/Anti-Viral Agent -
HY-13782
Tenofovir Disoproxil fumarateNucleotide Reverse Transcriptase Inhibitor -
HY-B0307
IdoxuridineAntiviral Agent -
HY-W011138
IbacitabineAntiviral Agent -
HY-W017265
5-Nitrouracil -
HY-14905
Uridine triacetateUridine Prodrug -
HY-W009163
5-BromouridineNucleoside -
HY-118122
FialuridineAntiviral Agent -
HY-13605A
Cytarabine hydrochlorideDNA Synthesis Inhibitor
Publications Citing Use of MedChemExpress (MCE) 1-beta-D-Arabinofuranosyluracil
More
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
>500 μM
Compound: AraU
|
Cytostatic activity human CEM cells
Cytostatic activity human CEM cells
|
[PMID: 20299228] |
| HL-60 | IC50 |
90 μM
Compound: AraU
|
Cytostatic activity against human HL60 cells
Cytostatic activity against human HL60 cells
|
[PMID: 20299228] |
| KG-1 | IC50 |
25 μM
Compound: AraU
|
Cytostatic activity human KG1 cells
Cytostatic activity human KG1 cells
|
[PMID: 20299228] |
| L1210 | IC50 |
170 μM
Compound: AraU
|
Cytostatic activity mouse L1210 cells
Cytostatic activity mouse L1210 cells
|
[PMID: 20299228] |
| NB-4 | IC50 |
69 μM
Compound: AraU
|
Cytostatic activity against human NB4 cells
Cytostatic activity against human NB4 cells
|
[PMID: 20299228] |
| U-937 | IC50 |
>100 μM
Compound: AraU
|
Cytostatic activity human U937 cells
Cytostatic activity human U937 cells
|
[PMID: 20299228] |
In Vitro
1-beta-D-Arabinofuranosyluracil (17 μM) reduces the proliferation of L5178Y mouse lymphoma cells[2].
1-beta-D-Arabinofuranosyluracil strongly inhibits RNA-directed DNA polymerases in L5178Y cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 3083-77-0
-
Appearance Solid
-
Molecular Weight 244.20
-
Formula C9H12N2O6
-
Color White to off-white
-
SMILES
O=C1NC(C=CN1[C@H]2[C@@H](O)[C@H](O)[C@@H](CO)O2)=O
-
Synonyms
Uracil 1-β-D-arabinofuranoside
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (204.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
-
Cotton Pellet Granuloma
Cotton pellet granuloma is a classical in vivo chronic inflammation model used to evaluate the anti-inflammatory potential of test substances by measuring their ability to inhibit granuloma tissue formation around an implanted foreign body (cotton pellet) in rodents. The method is based on the biological response to a sterile implanted material, which induces proliferative phase inflammation characterized by fibroblast proliferation and collagen-rich granuloma formation, and the final readout reflects the extent of chronic inflammatory tissue growth surrounding the pellet. In multiple preclinical pharmacological evaluations, inhibition of cotton pellet-induced granuloma formation has been used as an indicator of anti-inflammatory activity in both synthetic and natural product screening contexts.
-
Carrageenan-Induced Paw Edema
Carrageenan-induced paw edema is an acute inflammation model in which intraplantar injection of carrageenan induces localized inflammatory swelling characterized by vascular permeability, leukocyte infiltration, and production of inflammatory mediators such as prostaglandins and cytokines, making it widely used to evaluate anti-inflammatory agents in vivo. The resulting paw volume or thickness increase is quantified over time as a direct readout of inflammatory intensity and drug efficacy, typically reflecting cyclooxygenase-mediated prostaglandin-driven edema formation and immune cell recruitment in peripheral tissue[20].
-
Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
[1]. Bertin MJ, et al. Spongosine production by a Vibrio harveyi strain associated with the sponge Tectitethya crypta. J Nat Prod. 2015 Mar 27;78(3):493-9. [Content Brief]
[2]. Müller WE, et al. Metabolism of 1-beta-D-arabinofuranosyluracil in mouse L5178Y cells. Cancer Res. 1979 Mar;39(3):1102-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0950 mL | 20.4750 mL | 40.9500 mL | 102.3751 mL |
| 5 mM | 0.8190 mL | 4.0950 mL | 8.1900 mL | 20.4750 mL | |
| 10 mM | 0.4095 mL | 2.0475 mL | 4.0950 mL | 10.2375 mL | |
| 15 mM | 0.2730 mL | 1.3650 mL | 2.7300 mL | 6.8250 mL | |
| 20 mM | 0.2048 mL | 1.0238 mL | 2.0475 mL | 5.1188 mL | |
| 25 mM | 0.1638 mL | 0.8190 mL | 1.6380 mL | 4.0950 mL | |
| 30 mM | 0.1365 mL | 0.6825 mL | 1.3650 mL | 3.4125 mL | |
| 40 mM | 0.1024 mL | 0.5119 mL | 1.0238 mL | 2.5594 mL | |
| 50 mM | 0.0819 mL | 0.4095 mL | 0.8190 mL | 2.0475 mL | |
| 60 mM | 0.0683 mL | 0.3413 mL | 0.6825 mL | 1.7063 mL | |
| 80 mM | 0.0512 mL | 0.2559 mL | 0.5119 mL | 1.2797 mL | |
| 100 mM | 0.0410 mL | 0.2048 mL | 0.4095 mL | 1.0238 mL |
1-beta-D-Arabinofuranosyluracil Related Classifications
HY-N6652 Related Classifications
Powered by Bioz