10-Formyl-7,8-dihydrofolic acid

Cat. No.: HY-137129 Purity: 97.70%: Data Sheet
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10-Formyl-7,8-dihydrofolic acid is a biologically active folic acid and growth promoter. 10-Formyl-7,8-dihydrofolic acid serves as a substrate for aminoimidazolecarboxamide ribonucleotide transformylase and dihydrofolate reductase (DHFR) to support catalytic reactions (with detection wavelengths of 552 nm and 340 nm, respectively). 10-Formyl-7,8-dihydrofolic acid not only promotes the growth of leukemia cells, but also effectively reverses the growth inhibition induced by antifolate drugs under folate-deficient conditions. 10-Formyl-7,8-dihydrofolic acid can be used in the research of leukemia.

For research use only. We do not sell to patients.

10-Formyl-7,8-dihydrofolic acid Chemical Structure

CAS No. : 28459-40-7

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Description

In Vitro

10-Formyl-7,8-dihydrofolic acid (1-5000 nM; 90-162 h, with methotrexate co-treatment) reverses methotrexate-induced growth suppression in human acute lymphoblastic leukemia CCRF-CEM cells in a dose-dependent manner, with a mean fractional change in cell growth of 0.7, and supports increased cell viability at higher concentrations^[1].
10-Formyl-7,8-dihydrofolic acid (5 × 10^-4 mol/L; up to 300 min) is a substrate for dihydrofolate reductase from human acute lymphoblastic leukemia CCRF-CEM cells, supporting enzyme activity as measured by decreasing absorbance at 340 nm over time^[1].
10-Formyl-7,8-dihydrofolic acid (5 × 10^-4 mol/L; up to 24 h) is a substrate for aminoimidazole carboxamide ribotide transformylase from human acute lymphoblastic leukemia CCRF-CEM cells, supporting enzyme activity as measured by decreasing absorbance at 552 nm over time^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	human acute lymphoblastic leukemia CCRF-CEM cells
Concentration:	1 nM; 10 nM; 100 nM; 1000 nM; 5000 nM (with 5 nM/25 nM/100 nM Methotrexate)
Incubation Time:	90 h; 96 h; 97 h; 142 h; 162 h
Result:	Increased fractional change in cell number above control by 0.3 (96 h, 5 nmol/L methotrexate), 0.2 (142 h, 25 nmol/L methotrexate), 0.4 (162 h, 100 nmol/L methotrexate) with 32% viability at 1 nM. Increased fractional change in cell number above control by 0.2 (96 h, 5 nmol/L methotrexate), 0.3 (142 h, 25 nmol/L methotrexate), 0.4 (162 h, 100 nmol/L methotrexate) with 32% viability at 10 nM. Increased fractional change in cell number above control by 0.2 (96 h, 5 nmol/L methotrexate), 0.2 (97 h, 25 nmol/L methotrexate), 0.4 (142 h, 25 nmol/L methotrexate), 0.7 (90 h, 100 nmol/L methotrexate), 0.2 (162 h, 100 nmol/L methotrexate) with 43% viability at 100 nM. Increased fractional change in cell number above control by 0.3 (96 h, 5 nmol/L methotrexate), 0.6 (142 h, 25 nmol/L methotrexate), 2.8 (162 h, 100 nmol/L methotrexate) with 82% viability at 1000 nM. Increased fractional change in cell number above control by 0.3 (97 h, 25 nmol/L methotrexate), 1.2 (142 h, 25 nmol/L methotrexate), 3.6 (90 h, 100 nmol/L methotrexate), 8.2 (162 h, 100 nmol/L methotrexate) with 93% viability at 5000 nM. Achieved a mean fractional change in cell growth of 0.7.

Molecular Weight

471.42

Formula

C₂₀H₂₁N₇O₇

CAS No.

28459-40-7

Appearance

Solid

Color

Off-white to light brown

SMILES

O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(N(CC2=NC3=C(N=C(N)NC3=O)NC2)C=O)C=C1)=O

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Purity & Documentation

Purity: 97.70%

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Data Sheet (275 KB) SDS (419 KB)

COA (254 KB)

Handling Instructions (2659 KB)

References

[1]. Baggott JE, et al. 10-Formyl-dihydrofolic acid is bioactive in human leukemia cells. J Nutr. 1999;129(7):1315-1318. [Content Brief]